Liofilchem® Defibrinated Horse Blood

Liofilchem® Defibrinated Horse Blood is a high-quality sterile blood product specifically designed for use in microbiology laboratories. This reagent is essential for preparing enriched culture media and is particularly suited for cultivating a wide range of fastidious microorganisms. The blood is mechanically defibrinated to ensure it remains free from fibrin clots, offering consistent performance in microbial diagnostics and research.

Key Features

1. Mechanically Defibrinated:
   • Ensures no fibrin clots, providing a homogenous blood solution.
   • Optimized for media preparation and accurate microbial studies.
2. High Sterility Standards:
   • Prepared aseptically to minimize contamination risks.
   • Packaged in sterile bottles for safe and direct laboratory use.
3. Versatile Application:
   • Suitable for various microbiological applications, including pathogen isolation, susceptibility testing, and hemolysis studies.
4. Nutrient-Rich Composition:
   • Provides essential growth factors, including heme and other nutrients.
   • Supports fastidious microorganisms and hemolysis differentiation.
5. Available Sizes:
   • 50 mL (Cat. No. 83395): Ideal for small-scale applications or individual experiments.
   • 100 mL (Cat. No. 83396): Suited for larger-scale media preparation and routine laboratory use.

Applications

1. Microbiological Media Preparation:
   • Used as an additive to enrich culture media such as Blood Agar.
   • Ideal for creating chocolate agar when heated, supporting the growth of fastidious organisms like Haemophilus spp. and Neisseria spp.
2. Hemolysis Studies:
   • Essential for differentiating bacterial species based on hemolytic activity (alpha, beta, gamma hemolysis).
   • Commonly used for Streptococcus spp. and other hemolytic pathogens.
3. Pathogen Cultivation:
   • Supports the growth of fastidious microorganisms, including:
     • Streptococcus pneumoniae
     • Neisseria gonorrhoeae
     • Haemophilus influenzae
     • Brucella spp.
     • Pasteurella multocida
     • Corynebacterium diphtheriae
4. Veterinary Microbiology:
   • Specifically suited for studying zoonotic pathogens and veterinary diagnostics.
   • Enables the isolation and study of bacteria relevant to equine health.
5. Research and Pharmaceutical Use:
   • Widely employed in pharmaceutical quality control, environmental monitoring, and academic research.

Product Benefits

• Sterile and Ready-to-Use:
  • Simplifies laboratory workflow by eliminating the need for additional processing.
• Batch-to-Batch Consistency:
  • Ensures reliable results in experimental and diagnostic settings.
• Broad Compatibility:
  • Works with various microbiological protocols and media types.
• Safe Handling:
  • Aseptic packaging minimizes exposure to contaminants.

Usage Instructions

1. Storage:
   • Store the product at 2-8°C in its original sterile container.
   • Avoid exposure to heat or temperature fluctuations to maintain product integrity.
2. Media Preparation:
   • Add the desired volume of defibrinated horse blood to base media (e.g., 5% v/v for Blood Agar or Chocolate Agar).
   • Mix thoroughly to ensure even distribution.
3. Hemolysis Observation:
   • Incubate plates inoculated with test organisms and observe for hemolytic patterns:
     • Alpha (partial hemolysis, greenish zones).
     • Beta (complete hemolysis, clear zones).
     • Gamma (no hemolysis).

Quality Control

Each batch undergoes rigorous quality control to ensure sterility, performance, and absence of contaminants. The product is validated using reference strains such as:

• Streptococcus pyogenes (beta-hemolysis).
• Streptococcus pneumoniae (alpha-hemolysis).
• Escherichia coli (gamma-hemolysis).

Ordering Information

Storage and Shelf Life

• Temperature: 2-8°C.
• Shelf Life: Valid until the expiration date indicated on the label.
• Packaging: Aseptic, tamper-proof bottles to maintain sterility.

Precautions

• For in vitro diagnostic use only.
• Handle in a biosafety level-appropriate environment.
• Dispose of all used materials according to laboratory biohazard protocols.

Applications Across Industries

• Clinical microbiology laboratories.
• Veterinary diagnostic labs.
• Pharmaceutical quality control.
• Academic and industrial research facilities.

With its reliability and broad applicability, Liofilchem® Defibrinated Horse Blood is an essential tool for microbiologists focused on diagnostic accuracy and microbial studies.