Liofilchem® Amies Transport Medium w/ Charcoal

Liofilchem® Amies Transport Medium w/ Charcoal is a semi-solid, phosphate-buffered medium designed for the collection, transport, and preservation of microbiological specimens. It maintains the viability of microorganisms without promoting significant growth and is particularly suitable for anaerobic bacteria. The addition of charcoal enhances bacterial survival by acting as a detoxifying agent and reducing oxygen tension.

Key Features

1. Enhanced Preservation with Charcoal
   • Detoxifies the medium and reduces oxygen tension, improving bacterial survival.
2. Phosphate-Buffered, Semi-Solid Medium
   • Stabilizes pH during transport and reduces contamination risks.
3. Wide Applicability
   • Suitable for clinical specimens and a variety of microorganisms, including:
     • Haemophilus influenzae
     • Neisseria gonorrhoeae
     • Streptococcus pneumoniae
     • Streptococcus pyogenes
4. High Bacterial Viability
   • Supports gonococcal survival for up to 12 hours under controlled conditions.

Typical Formula (g/l)

Final pH: 7.2 ± 0.2 at 25°C

Preparation

1. Suspend 19.8 g of powder in 1 liter of distilled or deionized water.
2. Heat to boiling, stirring frequently until completely dissolved.
3. Dispense into final containers.
4. Sterilize in an autoclave at 121°C for 15 minutes.

Instructions for Use

1. Sample Collection
   • Use sterile swabs to collect specimens. Insert one-third of the swab into the medium.
   • Cut the stick and screw the cap tightly to submerge the swab.
   • Keep the specimen cool during transport.
2. Storage During Transport
   • Store at 2-8°C during the transport period.

Quality Control Data

Storage and Stability

• Dehydrated Powder: Store at 10-30°C in a dry environment, tightly sealed in its original container.
• Prepared Containers: Store at 2-8°C, protected from light.
• Shelf Life: 4 years for powder; 1 year for prepared medium.

Limitations

• Contaminants may grow during extended transport.
• Specimens should be inoculated promptly onto appropriate media.
• Not suitable for all fastidious organisms.
• Gonococcal survival decreases after 12 hours and may affect recovery if initially present in small numbers.

Packaging Options

Warnings and Precautions

1. For in vitro diagnostic use (IVD) only.
2. Intended for use by trained laboratory professionals.
3. Follow safety precautions as per institutional protocols.
4. Dispose of waste according to local and national regulations.

Amies Transport Medium w/ Charcoal ensures specimen viability during transport and is a vital tool for reliable microbiological diagnostics.