Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 μm, 8.5 x 13.5 cm

Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches offer high protein binding capacity and robust performance for Western blotting, dot blot assays, and protein sequencing workflows. Each sandwich includes a 0.45 µm PVDF membrane and extra-thick 2.5 mm filter paper, pre-assembled and ready for rapid setup in semi-dry or traditional transfer systems.

Optimized for the efficient transfer of proteins >10 kDa, the PVDF membrane provides superior durability, compatibility with a wide range of detection methods, and the ability to be stripped and re-probed. The pre-cut format eliminates the need for manual trimming, reducing preparation time and improving blotting consistency.

Key Features and Benefits

• Ready-to-use pre-cut sandwiches – simplify and speed up Western blot setup
• High binding capacity – ideal for detection of proteins in the low ng range
  • Goat IgG: ~294 µg/cm²
  • BSA: ~131 µg/cm²
  • Insulin: ~85 µg/cm²
• 0.45 µm pore size – optimal for transferring proteins >10 kDa
• Durable PVDF membrane – compatible with organic solvents, acids, and mild bases
• Re-probable – membrane can be stripped and reused for additional analysis
• Pre-activation required – activate in 100% methanol before use
• Thick filter paper (2.5 mm) – enhances buffer capacity and ensures even transfer
• Versatile detection compatibility – compatible with chemiluminescence, chromogenic, fluorescence, and radiographic detection

Applications

• Western blotting of proteins >10 kDa
• Dot blot assays
• Protein sequencing and identification
• Chemiluminescence and fluorescence detection
• Reprobing and multiplex immunoblotting

Technical Specifications

Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches are an efficient and reliable solution for high-quality protein transfers in Western blotting and protein analysis workflows. With a high binding capacity, pre-assembled convenience, and broad compatibility with detection techniques, these membrane sandwiches are ideal for consistent, reproducible blotting results in both high-throughput and routine applications.