SinoBio Lentivirus (HIV-1 p24) Titer Kit

The SinoBio Lentivirus (HIV-1 p24) Titer Kit is a ready-to-use enzyme-linked immunosorbent assay (ELISA) designed for the quantitative detection and measurement of HIV-1 Gag-p24 protein in recombinant protein samples. This kit utilizes a solid-phase sandwich ELISA format to provide accurate and reliable results with high sensitivity and specificity.

Key Features:

1. Assay Type:
   • Solid Phase Sandwich ELISA for quantitative analysis.
2. Target Molecule:
   • HIV-1 Gag-p24 protein.
3. Sensitivity and Range:
   • Sensitivity: 9.90 pg/mL.
   • Dynamic Range: 31.25–2000 pg/mL.
4. Specificity:
   • Recognizes recombinant HIV-1 p24 protein with high precision.
5. Conjugate:
   • Horseradish Peroxidase (HRP)-conjugated detection antibody.

Applications:

• Lentivirus Quantification:
  • Determine lentivirus titers during viral production for transduction experiments.
• Viral Packaging Validation:
  • Monitor viral packaging and particle concentration for gene delivery research.
• Recombinant Protein Analysis:
  • Measure HIV-1 Gag-p24 in recombinant protein preparations.
• HIV-1 Research:
  • Suitable for investigating HIV-1 pathogenesis, virology, and lentivirus-based vector systems.

Kit Components:

1. 96-Well Microplate:
   • Pre-coated with capture antibody specific for HIV-1 Gag-p24 protein.
2. Detection Antibody:
   • Conjugated with HRP for signal amplification.
3. Standards:
   • Recombinant HIV-1 Gag-p24 protein for standard curve preparation.
4. Buffers and Reagents:
   • Wash Buffer Concentrate.
   • Dilution Buffer Concentrate.
   • Color Reagent A and B (for colorimetric detection).
   • Stop Solution to terminate the reaction.

Shipping and Storage:

• Shipping Condition:
  • Shipped at ambient temperature to maintain reagent integrity during transit.
• Storage:
  • Unopened Kit: Store at 2–8°C.
  • Opened/Reconstituted Reagents: Refer to the Certificate of Analysis (CoA) for specific storage instructions.

Assay Procedure Overview:

1. Sample Preparation:
   • Dilute recombinant protein samples using the dilution buffer provided.
2. Standard Curve Preparation:
   • Prepare a standard curve using the recombinant HIV-1 Gag-p24 standards supplied.
3. Capture and Incubation:
   • Add samples and standards to the pre-coated microplate and incubate.
4. Detection:
   • Add HRP-conjugated detection antibody, followed by washing steps.
5. Color Development:
   • Add Color Reagents A and B to initiate a colorimetric reaction.
6. Stop Reaction:
   • Terminate the reaction using the Stop Solution.
7. Read Results:
   • Measure absorbance at 450 nm using a microplate reader.

Advantages:

1. High Sensitivity and Accuracy:
   • Sensitivity as low as 9.90 pg/mL ensures precise quantification of low-abundance HIV-1 Gag-p24 protein.
2. Comprehensive Kit:
   • Includes all necessary reagents for easy and efficient setup.
3. Versatility:
   • Suitable for a wide range of applications in virology, lentivirus research, and gene therapy.
4. User-Friendly Format:
   • Pre-coated microplate and ready-to-use reagents minimize setup time.

Use Cases:

1. Lentiviral Vector Development:
   • Quantify lentivirus titers for transduction optimization in gene therapy applications.
2. HIV-1 Research:
   • Investigate HIV-1 replication dynamics and viral assembly processes.
3. Recombinant Protein Quality Control:
   • Assess the concentration and quality of recombinant HIV-1 p24 protein in production.
4. Biotech and Pharmaceutical Applications:
   • Monitor lentiviral products in R&D and production environments.

The SinoBio Lentivirus (HIV-1 p24) Titer Kit provides a robust, sensitive, and reliable solution for quantifying HIV-1 Gag-p24 protein. Its comprehensive kit design and precise performance make it an ideal tool for virology and lentivirus-based research.