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Applied Biosystems™ PicoPure™ DNA Extraction Kit for 10 – 150 Preps
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Specifications:
| Application | Nucleic Acid Extraction |
| Storage Temperature | Room Temperature |
| Product Type | Sample Prep |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The ARCTURUS™ PicoPure™ DNA Extraction Kit is an efficient, reliable, and streamlined solution for genomic DNA extraction from as few as 10 cells. Designed for research applications, this kit eliminates the need for spin columns or organic extractions, enabling direct, PCR-ready DNA recovery for use in various downstream applications such as real-time PCR, mutation analysis, and genotyping assays.
Key Features
- Streamlined Workflow:
- Simplifies genomic DNA extraction for PCR-ready samples.
- Extract and amplify DNA in the same tube without additional purification steps.
- High Efficiency:
- Reliable and reproducible DNA recovery from as few as 10 cells or tissue sections.
- Maximizes DNA recovery by eliminating loss from organic extractions or spin columns.
- Versatile Applications:
- Compatible with real-time PCR, endpoint PCR, mutation analysis, and genotyping.
- Suitable for DNA extraction from Laser Capture Microdissection (LCM) samples, FFPE tissue, and frozen tissue.
- Flexible Compatibility:
- Works with a wide range of tissue and cell preparation methods, including ethanol-fixed cells and cytological smears.
- Convenient Kit Components:
- Includes lyophilized Proteinase K and PCR-compatible Reconstitution Buffer for simple, stable, and reproducible use.
Specifications
| Parameter | Details |
|---|---|
| Format | Tube |
| Isolation Technology | Enzymatic PCR Clean-up Technology |
| Sample Type | LCM, FFPE, Frozen Tissue, Ethanol-fixed cells |
| Final Product Type | Genomic DNA |
| For Use With (Equipment) | ArcturusXT™ LCM Instrument |
| Applications | Real-Time Quantitative PCR (qPCR), PCR |
| No. of Reactions | 10–150 Preps |
| Product Line | PicoPure™ |
| Target | Genomic DNA |
| Starting Material | As few as 10 cells |
| High-throughput Compatibility | Manual, not high-throughput compatible |
| Storage | Room temperature |
| Contents | Lyophilized Proteinase K, Reconstitution Buffer |
Applications
- PCR-Ready DNA Extraction:
- Direct extraction and amplification for gene copy number quantitation and mutation analysis.
- Real-Time PCR:
- Ideal for sensitive qRT-PCR applications using minimal starting material.
- Genotyping and Mutation Analysis:
- Compatible with downstream workflows like dHPLC (e.g., WAVE™ System by Transgenomics).
- Tissue and Cell Analysis:
- Effective for FFPE tissue, frozen tissue sections, and cells processed by LCM.
Advantages
| Feature | Benefit |
|---|---|
| Direct PCR Workflow | Reduces steps by enabling extraction and PCR in the same tube. |
| High Sensitivity | Recovers DNA from as few as 10 cells, enhancing detection sensitivity. |
| Reproducible Results | Ensures consistent DNA recovery without sample loss. |
| Versatility | Compatible with a range of sample preparation methods. |
| No Organic Extractions Needed | Simplifies workflows and eliminates hazardous waste. |
Limitations
- Sample Compatibility: Not recommended for whole blood, plant, or fungal samples.
- Manual Process: Not suitable for high-throughput workflows.
Kit Contents
- Lyophilized Proteinase K: Stable and easy to reconstitute for DNA extraction.
- Reconstitution Buffer: PCR-compatible for seamless downstream applications.
- User Guide: Detailed instructions for efficient DNA recovery.
Sample Compatibility
| Sample Type | Examples |
|---|---|
| Laser Capture Microdissection | CapSure™ HS LCM Cap, CapSure™ Macro Cap |
| Formalin-Fixed Paraffin-Embedded (FFPE) | Archived tissue samples |
| Frozen Tissue Sections | Snap-frozen or ethanol-fixed tissues |
| Ethanol-Fixed Cells | Cytological smears, ethanol-preserved cells |
Performance Comparison
- PicoPure™ DNA Extraction Kit vs Leading Manufacturer DNA Extraction Kits:
- Higher Recovery: Amplifies earlier in qRT-PCR, indicating more DNA is present.
- Consistent Results: Reduces sample variation, ensuring reproducibility.
For Research Use Only
The PicoPure™ DNA Extraction Kit is intended for research purposes only and is not suitable for diagnostic or clinical procedures.
ARCTURUS™ PicoPure™ DNA Extraction Kit is an ideal solution for researchers requiring efficient, reliable, and reproducible DNA recovery from small samples for PCR applications.