Invitrogen™ Human CRP ELISA Kit

The Invitrogen™ Human C-Reactive Protein (CRP) ELISA Kit is a sensitive and specific assay designed to quantitatively measure CRP levels in human serum and plasma. This kit exclusively recognizes both natural and recombinant human CRP and is essential for research into inflammatory responses, immune system regulation, and cardiovascular disease.

Principle of the Method

The assay employs a solid-phase sandwich ELISA technique to measure CRP levels:

1. Pre-Coated Plate:
   • Microplate wells are pre-coated with a target-specific capture antibody.
2. Sample and Standard Addition:
   • Samples, standards, or controls are added to the wells and bind to the immobilized capture antibody.
3. Detection Antibody Addition:
   • A biotinylated detector antibody specific to CRP is added to form a sandwich complex.
4. Signal Development:
   • Streptavidin-HRP and a substrate solution are added, producing a measurable colorimetric signal proportional to the CRP concentration.
5. Measurement:
   • The intensity of the color is measured at 450 nm using a colorimetric microplate reader.

Key Features

1. Rigorous Validation:
   • Every lot undergoes quality testing for sensitivity, specificity, precision, and consistency.
2. High Sensitivity:
   • Analytical sensitivity of <10 pg/mL.
3. Wide Assay Range:
   • Dynamic range of 18.75-1200 pg/mL.
4. Precision:
   • Interassay CV: <9.7%, Intraassay CV: <7%.
5. Ease of Use:
   • Hands-on time of 1 hr 20 min with time-to-result of 4 hr.

Applications

• Inflammation Research:
  • Study CRP as a biomarker in inflammatory and infectious diseases.
• Cardiovascular Disease Research:
  • Investigate CRP’s role in hypertension and cardiovascular risk.
• Immune Regulation Studies:
  • Explore CRP's function in innate immunity and complement activation.
• Infectious Disease Research:
  • Assess CRP levels in response to bacterial and viral infections.
• Therapeutic Development:
  • Evaluate CRP as a target for anti-inflammatory therapies.

Specifications

• Analytical Sensitivity: <10 pg/mL
• Assay Range: 18.75-1200 pg/mL
• Sample Type/Volume:
  • Plasma: 5 µL
  • Serum: 5 µL
• Hands-On Time: 1 hr 20 min
• Time-to-Result: 4 hr
• Instrument: Colorimetric Microplate Reader
• Interassay CV: <9.7%
• Intraassay CV: <7%
• Product Size: 96 Tests

Kit Contents

• Pre-coated 96-well plate
• CRP Standard
• Standard Dilution Buffer
• Biotinylated Detection Antibody
• Streptavidin-HRP
• HRP Diluent
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers

Target Information

CRP (C-Reactive Protein):

• Structure: Pentameric acute phase protein; five identical nonglycosylated subunits.
• Molecular Weight: ~24 kDa per subunit.
• Production: Synthesized by the liver in response to inflammation.
• Functions:
  • Initiates classical complement cascade.
  • Acts as an opsonin and activates monocytes/macrophages.
  • Binds to nuclear components like chromatin and histones.
  • Plays a critical role in innate immunity and pathogen elimination.
• Relevance:
  • Elevated CRP levels are indicative of inflammation, infection, or injury.
  • CRP levels correlate with cardiovascular disease and hypertension risks.

Storage and Stability

• Storage Conditions: Store all components at 2–8°C.
• Shipping Conditions: Wet ice.
• Stability: Ensure components are stored properly to maintain reagent integrity.

The Invitrogen™ Human CRP ELISA Kit is an indispensable tool for researchers studying inflammation, immune responses, and cardiovascular disease. Its high sensitivity, specificity, and rigorous validation ensure accurate and reproducible results in clinical and experimental applications.