Invitrogen™ SAA Human ELISA Kit

The Invitrogen™ Human Serum Amyloid A (SAA) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of human SAA levels in serum, plasma, buffered solutions, and cell culture media. This kit recognizes both the natural 18 kDa isoform and recombinant SAA, making it essential for research into inflammatory responses, acute-phase reactions, and disease monitoring.

Principle of the Method

The assay employs a solid-phase sandwich ELISA technique for accurate quantification of SAA levels:

1. Pre-Coated Plate:
   • Microplate wells are pre-coated with a capture antibody specific to SAA.
2. Sample and Standard Addition:
   • Samples, standards, or controls are added to the wells, where SAA binds to the immobilized capture antibody.
3. Detection Antibody Addition:
   • A detector antibody forms a sandwich complex with the target SAA.
4. Signal Development:
   • A substrate solution is added that reacts with the enzyme-antibody-target complex, producing a measurable colorimetric signal proportional to the SAA concentration.
5. Measurement:
   • The signal intensity is measured at 450 nm using a colorimetric microplate reader.

Key Features

1. Rigorous Validation:
   • Each lot undergoes quality testing for sensitivity, specificity, precision, and consistency.
2. High Sensitivity:
   • Measures low levels of SAA with precision.
3. Specificity:
   • Recognizes both natural and recombinant SAA.
4. Ease of Use:
   • Pre-coated plates and ready-to-use reagents simplify workflows.

Applications

• Inflammation Research:
  • Study SAA as an acute-phase biomarker in inflammatory conditions.
• Disease Monitoring:
  • Evaluate SAA levels for tracking disease progression and therapeutic efficacy.
• Cancer Research:
  • Investigate SAA’s role in tumor-associated inflammation and metastasis.
• Infectious Disease Research:
  • Assess SAA levels during bacterial and viral infections.
• Autoimmune Studies:
  • Explore SAA’s involvement in autoimmune disorders and chronic inflammatory diseases.

Target Information

Serum Amyloid A (SAA):

• Function:
  • SAA is an acute-phase apolipoprotein synthesized primarily in the liver during inflammation.
  • Plays a critical role in immune responses and tissue repair.
• Characteristics:
  • SAA levels can increase by up to 1000-fold during inflammation.
  • Its expression and function are being explored in broader disease contexts.
• Clinical Importance:
  • Rises earlier and more sharply than CRP, making it a sensitive marker for acute-phase reactions.

Kit Specifications

• Sample Types:
  • Serum, plasma, buffered solution, and cell culture media.
• Target Recognition:
  • Natural 18 kDa isoform and recombinant SAA.
• Usage:
  • For research use only, not for diagnostic procedures.

Kit Contents

• Pre-coated microplate
• Standards
• Biotinylated detection antibody
• Streptavidin-HRP
• Wash buffer
• Chromogen
• Stop solution
• Adhesive plate covers

Storage and Stability

• Storage Conditions: Store all kit components at 2–8°C.
• Shelf Life: Stable under proper storage conditions.

The Invitrogen™ Human SAA ELISA Kit is an essential tool for researchers studying inflammation, acute-phase reactions, and disease monitoring. Its high sensitivity, rigorous validation, and broad sample compatibility make it ideal for clinical and experimental research applications.