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Invitrogen™ PureLink™ FFPE RNA Isolation Kit, 5 Preps
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Specifications:
| Application | RNA Extraction | ||
| Storage Temperature | Room Temperature, -20°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ FFPE RNA Isolation Kit provides a reliable and efficient method for isolating high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Designed specifically for retrospective gene expression studies, this kit eliminates the need for toxic organic solvents like xylene, instead employing a simple heating procedure for deparaffinization and RNA recovery.
With a streamlined workflow, RNA isolation can be completed in 30–40 minutes, compared to days with older methods. The heating step partially restores RNA structure, improving template quality for cDNA synthesis and downstream RT-PCR applications.
Key Features
- Fast Protocol – RNA extraction completed in 30–40 minutes.
- Non-Toxic Workflow – Deparaffinization by heating, no xylene or hazardous solvents.
- High Yield & Purity – Recovers RNA suitable for RT-PCR products >1 kb in length.
- Low DNA Contamination – Minimizes genomic DNA carryover; includes DNase I option.
- Reliable Results – Produces RNA with maximum yield and minimal degradation.
Applications
- Reverse transcription (RT).
- Real-time PCR (qPCR) and qRT-PCR.
- Microarray analysis.
- cDNA library construction.
- Northern blotting, nuclease protection assays, and in vitro translation.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | FFPE RNA Isolation Kit |
| Catalog Number | K156002 |
| Quantity | 50 preps |
| Sample Input | 3–8 sections of 10 µm FFPE tissue |
| Yield | >1 µg RNA |
| Elution Volume | 50 µL |
| Test Time | 30–40 minutes |
| Isolation Technology | Silica spin column |
| Scale | Mini |
| Throughput | Manual (not high-throughput) |
| Shipping Condition | Box 1: Ice; Box 2: Room temperature |
| Storage | Buffers at RT; DNase I at –20 °C |
| Unit Size | Each kit (50 preps) |
Kit Contents
- Melting Buffer
- Proteinase K
- Binding Buffer L3
- Wash Buffer W5
- RNase-free Water
- DNase I (amplification grade, optional)
- Spin cartridges with collection tubes
- Wash tubes and recovery tubes
Unique Advantages
- Eco-friendly compared to solvent-based protocols.
- Recovers longer RNA fragments (>1 kb) than competitor kits.
- Optimized for retrospective studies from archived FFPE specimens.
- Trusted Invitrogen quality ensures reproducibility in downstream applications.
The Invitrogen™ PureLink™ FFPE RNA Isolation Kit is a fast, safe, and efficient system for extracting total RNA from FFPE tissues. By eliminating hazardous solvents, delivering higher yields, and providing RNA suitable for RT-PCR, qPCR, and microarray studies, it is an indispensable tool for researchers working with archival tissue specimens.