Invitrogen™ UltraPure™ Buffer-Saturated Phenol

The Invitrogen™ UltraPure™ Buffer-Saturated Phenol is a molecular biology-grade reagent designed for the efficient purification of nucleic acids. Saturated with Tris-HCl buffer at pH >7.4, this solution ensures optimal denaturation of proteins, facilitating the separation of nucleic acids into the aqueous phase during extraction workflows. The product is rigorously tested to ensure the absence of RNase and DNase activity.

Key Features

1. Pre-Equilibrated to pH >7.4:
   • Buffer-saturated with Tris-HCl for DNA purification workflows requiring alkaline conditions.
2. Efficient Phase Separation:
   • Proteins are denatured and collected in the organic phase or interphase, while nucleic acids remain in the aqueous phase.
3. Preservative-Free:
   • Contains no preservatives, ensuring compatibility with sensitive molecular biology applications.
4. High-Quality Packaging:
   • Supplied in shatter-resistant, plastic-coated amber bottles under an inert gas to protect the reagent from light and oxidation.
5. Rigorous Quality Control:
   • Tested to confirm the absence of RNase and DNase activity, preserving nucleic acid integrity.

Specifications

Applications

1. DNA Extraction:
   • Separate nucleic acids from proteins during purification workflows.
2. RNA Extraction:
   • Ideal for RNA isolation protocols requiring phenol-based reagents.
3. Nucleic Acid Sample Purification:
   • Remove proteins and other contaminants from nucleic acid preparations.
4. Phase Separation Workflows:
   • Used in conjunction with chloroform for improved phase separation in phenol-based extractions.

Advantages

Comparison with Competitor Products

Workflow Overview

1. Sample Preparation:
   • Homogenize the biological sample in an appropriate buffer.
2. Add UltraPure™ Buffer-Saturated Phenol:
   • Add an equal volume of phenol to the sample and mix thoroughly.
3. Phase Separation:
   • Centrifuge the sample to separate the aqueous and organic phases.
4. Recovery:
   • Collect the aqueous phase containing nucleic acids for downstream processing.
5. Storage:
   • Store the purified nucleic acids at -20°C or -80°C.

Suggested Pairing

• Chloroform:
  • Use with Invitrogen™ UltraPure™ Phenol for enhanced performance in phase separation.
• Downstream Applications:
  • Combine with Invitrogen™ Platinum™ PCR reagents for amplification of purified DNA.
• RNA Isolation:
  • Pair with TRIzol™ Reagent for comprehensive RNA purification workflows.

The Invitrogen™ UltraPure™ Buffer-Saturated Phenol is a reliable, high-performance reagent for efficient nucleic acid purification, trusted by researchers for its ease of use, consistent results, and compatibility with sensitive molecular biology applications.