Invitrogen™ Superscript™ Iii One-Step Rt-Pcr System With Platinum™ Taq Dna Polymerase, 100 Rxn

The SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase offers a high-performance solution for sensitive and reproducible end-point RT-PCR detection and analysis of RNA molecules. This system combines cDNA synthesis and PCR amplification in a single-tube, streamlined workflow, reducing hands-on time and minimizing contamination risk. Ideal for detecting targets from total RNA or mRNA, the system allows researchers to work efficiently with even low-abundance RNA templates.

Key Features

• Single-Tube Workflow: Combines reverse transcription and PCR amplification in one step, reducing variability and workflow complexity.
• High Sensitivity: Detects as little as 0.01 pg of total RNA.
• Wide Target Range: Amplifies RNA targets from 200 bp to 4.5 kb.
• High Specificity: Reverse transcription up to 55°C, reducing nonspecific priming.
• Hot Start PCR: Platinum Taq is antibody-inhibited until heat activation, enhancing specificity and yield.

System Components

• SuperScript III RT/Platinum Taq Mix (200 μL) – high-performance enzyme mix for cDNA synthesis and amplification.
• 2X Reaction Mix (3 x 1 mL) – optimized buffer for efficient RT-PCR.
• 5 mM Magnesium Sulfate (500 μL) – for fine-tuning Mg²⁺ concentration based on experimental needs.

Enzymes & Mechanism

SuperScript III Reverse Transcriptase

• Engineered from M-MLV RT with reduced RNase H activity.
• High thermal stability allows reverse transcription up to 60°C.
• Tolerates rRNA and tRNA, ideal for total RNA inputs.

Platinum Taq DNA Polymerase

• Recombinant Taq DNA polymerase bound to a proprietary antibody.
• Automatic Hot Start mechanism activated at 94°C for improved PCR fidelity and yield.

Applications

• Endpoint detection of gene expression
• Viral RNA detection
• Screening assays
• Molecular biology research
• Pre-clinical diagnostics (RUO)

Specifications

Ordering Information

Note: For real-time qPCR, use the SuperScript™ III Platinum™ One–Step Quantitative RT-PCR System.

Storage

Store all components at –10°C to –30°C. Avoid repeated freeze-thaw cycles to preserve enzyme activity.

Regulatory Status

• For Research Use Only. Not intended for use in diagnostic procedures.