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Invitrogen™ SimplyBlue™ SafeStain, 1X ready-to-use
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Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Gel Stain | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
SimplyBlue™ SafeStain from Invitrogen™ is a pre-formulated, non-hazardous Coomassie G-250 stain designed for sensitive and safe visualization of protein bands in polyacrylamide gels and on dry PVDF membranes. It eliminates the need for methanol and acetic acid, making it ideal for labs that prioritize safety, simplicity, and compatibility with mass spectrometry or other downstream analyses.
With a simplified protocol, no fixation or destaining required, and microwave compatibility for ultra-fast workflows, SimplyBlue™ SafeStain is an ideal choice for routine and high-throughput SDS-PAGE applications.
Key Features and Benefits
- Ready-to-use 1X formulation—no mixing or dilution required
- Non-hazardous—no methanol or acetic acid needed
- Fast protocol—stain visible in as little as 5 minutes
- Microwave-compatible—12-minute fast staining option
- High sensitivity—detect as little as 7 ng of protein
- No destaining needed—but optional water wash enhances clarity for mass spec
- Environmentally friendly—safer for lab personnel and reduced disposal concerns
- Compatible with polyacrylamide gels and dry PVDF membranes
Applications
- SDS-PAGE protein staining
- Protein purity assessment
- Gel documentation and band excision for mass spectrometry
- General proteomics workflows
- Visualization prior to Western blotting or electroelution
Protocol Options
- Standard Stain: <3 hours total, no fixation or destaining required
- Microwave Protocol: 12 minutes for rapid results
- Optional Water Wash: For enhanced sensitivity and clean background when needed
Package Sizes
| Catalog Number | Volume | Format |
|---|---|---|
| LC6060 | 1 L | 1X Ready-to-Use |
| LC6065 | 3.5 L | 1X Ready-to-Use |
Storage & Stability
- Store at room temperature
- Stable for 6 months when sealed and properly stored
- No hazardous materials shipping or storage requirements
SimplyBlue™ SafeStain delivers rapid, sensitive, and safe protein visualization without compromising lab safety or downstream performance. Designed for researchers who demand efficiency, non-toxicity, and reproducibility, it provides a modern Coomassie staining alternative to traditional harsh chemical protocols.
- Pack Size: Plastic bottle, 1L Plastic bottle, 3.5L