Invitrogen™ Second Strand cDNA Synthesis Kit

The Invitrogen™ Second Strand cDNA Synthesis Kit is a streamlined and efficient solution for generating high-quality, double-stranded complementary DNA (cDNA) from first-strand cDNA templates. Designed for molecular biology workflows, this kit eliminates the need for intermediate purification steps, such as organic extraction or ethanol precipitation, allowing for faster and higher-yielding cDNA synthesis. It is ideal for researchers focused on full-length cDNA cloning, double-stranded cDNA library construction, and transcriptome analysis.

Key Features and Benefits

• Rapid Protocol
  Complete second-strand synthesis within one hour, minimizing hands-on time and enabling faster downstream applications.
• Single-Tube Reaction Format
  All enzymatic reactions are performed in a single tube, simplifying the workflow and reducing the risk of sample loss or contamination.
• Pre-Mixed Components
  The kit comes with pre-aliquoted, ready-to-use enzyme mixes and buffers, reducing pipetting steps and improving reproducibility.
• Efficient RNA Removal
  Includes RNase I and EDTA to eliminate residual RNA from the first-strand reaction, ensuring high-purity double-stranded cDNA.
• High Recovery and Yield
  Optimized formulation ensures maximum conversion of first-strand cDNA into full-length, blunt-ended double-stranded cDNA suitable for cloning and sequencing.

Applications

• Double-Stranded cDNA Library Construction
  Enables the preparation of representative cDNA libraries from total RNA or mRNA.
• Full-Length cDNA Cloning
  Generates blunt-end double-stranded cDNA ideal for downstream ligation and vector cloning.
• Gene Expression Studies and Transcriptomics
  Suitable for applications requiring accurate representation of transcript variants or full-length gene products.

Technical Specifications

Ordering Information

The Invitrogen™ Second Strand cDNA Synthesis Kit offers a highly efficient, user-friendly method for synthesizing double-stranded cDNA from first-strand products. With its simplified, single-tube workflow and pre-formulated reagents, this kit is an ideal choice for researchers involved in gene expression analysis, cDNA library construction, and full-length gene cloning. The kit’s robust performance ensures accurate transcript representation and seamless integration into downstream cloning and sequencing workflows.