Invitrogen™ Platinum™ Hot Start PCR Master Mix (2X)

The Invitrogen™ Platinum™ Hot Start PCR Master Mix (2X) is a ready-to-use PCR reagent solution containing Platinum Taq DNA Polymerase, optimized PCR buffer, magnesium, and dNTPs for high-specificity DNA amplification. Designed with antibody-mediated hot-start technology, this master mix prevents nonspecific amplification during reaction setup and improves yield and reproducibility.

Available in both colorless and green formats, this master mix simplifies PCR setup, reduces pipetting steps, and supports reliable amplification across a broad range of DNA targets, including GC-rich templates.

Key Features

• Antibody-mediated Hot Start PCR technology for high specificity
• Ready-to-use 2X master mix with polymerase, MgCl₂, buffer, and dNTPs
• Minimizes nonspecific amplification and primer-dimer formation
• Convenient room temperature reaction setup
• High robustness and reproducibility
• Supports amplification of targets up to 5 kb
• Includes Platinum GC Enhancer for improved GC-rich amplification
• Green version allows direct gel loading without loading dye

Available Formats

Colorless Master Mix (Recommended for fluorescence-based detection)

Green Master Mix (Includes loading dye for direct gel loading)

Hot Start PCR Technology

Platinum Taq DNA Polymerase is complexed with proprietary antibodies that inhibit enzyme activity at lower temperatures. This prevents premature amplification and nonspecific binding during reaction setup. During the initial denaturation step, the antibodies are inactivated, restoring full polymerase activity for efficient and specific DNA amplification.

Applications

• Hot Start PCR amplification
• Genotyping assays
• Gene expression analysis
• Next-generation sequencing (NGS) workflows
• Routine molecular biology PCR applications
• Amplification of GC-rich DNA targets

Technical Specifications

Kit Contents

Each kit includes:

• Platinum Hot Start PCR Master Mix (2X)
• Platinum GC Enhancer
• Nuclease-free water

Color Format Selection Guide

The Invitrogen™ Platinum™ Hot Start PCR Master Mix (2X) provides a reliable, high-performance solution for DNA amplification with superior specificity and convenience. Its antibody-mediated hot-start mechanism, optimized buffer system, and ready-to-use format make it ideal for routine PCR, genotyping, and molecular biology applications in research laboratories.