Invitrogen™ Permeabilization Medium (Medium B)

The Invitrogen™ Permeabilization Medium (Medium B) is a vital component of the FIX & PERM™ Cell Permeabilization Kit, specifically designed for flow cytometry applications. This reagent enables permeabilization of cells in suspension, granting antibodies access to intracellular targets while preserving cell morphology and scatter characteristics. It is ideal for simultaneous detection of intracellular and surface markers, offering flexibility and precision for intracellular staining protocols.

Key Features:

1. Efficient Permeabilization:
   • Allows access to intracellular targets for antibody binding without disrupting cell scatter patterns.
2. Reduced Background Staining:
   • Specially formulated to minimize non-specific staining, enhancing signal clarity.
3. Simultaneous Staining:
   • Compatible with fluorochrome-labeled antibodies for intracellular staining during the permeabilization step.
4. Flexible Application:
   • Works with a wide range of biological samples, including cell lines, whole blood, and bone marrow.
5. Component of FIX & PERM™ Kit:
   • Used in combination with Fixation Medium (Medium A) for a complete fixation and permeabilization workflow.

Specifications:

Applications:

1. Flow Cytometry:
   • Permeabilizes cells for intracellular staining of cytokines, transcription factors, and other intracellular proteins.
2. Intracellular Cytokine Staining:
   • Enables detection of cytokines such as IFN-γ, IL-2, and IL-17A in immune cells.
3. Simultaneous Staining:
   • Facilitates simultaneous staining of surface and intracellular markers for complex multicolor panels.
4. Sample Types:
   • Suitable for use with diverse biological samples, including whole blood, bone marrow, and cultured cell lines.
5. Immunophenotyping:
   • Ideal for staining immune cell subsets with intracellular and surface markers.

Key Benefits:

1. Minimizes Background:
   • Specially formulated to reduce non-specific staining, enhancing signal clarity for intracellular staining.
2. Maintains Cell Morphology:
   • Leaves scatter characteristics intact, ensuring accurate gating during flow cytometric analysis.
3. Simplifies Protocols:
   • Supports simultaneous staining of intracellular and surface markers, streamlining workflows.
4. Flexible and Compatible:
   • Validated for use with a broad range of fluorochrome-labeled antibodies and sample types.
5. Easy to Use:
   • Pre-formulated and ready-to-use for intracellular staining protocols.

How It Works:

1. Fixation Step:
   • Cells are fixed with Fixation Medium (Medium A) for 15 minutes at room temperature.
2. Permeabilization and Staining Step:
   • Cells are washed and incubated with Permeabilization Medium (Medium B) along with fluorochrome-labeled antibodies for intracellular staining.
3. Analysis:
   • Analyze cells using flow cytometry, ensuring accurate detection of intracellular markers.

Storage and Handling:

• Store at room temperature for long-term stability.
• Protect from extreme temperatures to maintain reagent efficacy.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.