Invitrogen™ Nitrocellulose/Filter Paper Sandwiches, 0.2 μm, 8.5 x 13.5 cm

Invitrogen™ Nitrocellulose/Filter Paper Sandwiches are high-quality, ready-to-use membrane assemblies designed for efficient electrophoretic transfer of low molecular weight proteins (<20 kDa) and nucleic acids (<300 bp) in Western blotting, Southern/Northern blotting, and other nucleic acid detection methods.

This specific configuration features a 0.2 μm and 0.45 μm pore sizes nitrocellulose membrane combined with extra-thick 2.5 mm absorbent blotting paper, pre-assembled into a convenient 8.5 × 13.5 cm sandwich format. Ideal for semi-dry transfer systems, this format provides consistent and uniform contact, streamlining membrane setup while preserving binding efficiency and compatibility with multiple detection techniques.

Key Features and Benefits

• 0.2 μm and 0.45 μm pore size – optimized for small proteins and nucleic acids, reducing loss of low MW analytes
• Pre-assembled format – saves time and reduces handling variability during blot setup
• Extra-thick absorbent paper (2.5 mm) – enhances buffer distribution in semi-dry transfer systems
• Strong protein binding (~80 µg/cm²) – suitable for low-abundance target detection
• Versatile compatibility – supports chemiluminescence, fluorescence, radiolabeling, and colorimetric detection
• Hydrophobic and electrostatic interactions – ensure stable protein or nucleic acid immobilization
• Convenient dimensions – ideal for midi-sized gels and blotting formats

Product Variants

Invitrogen™ Nitrocellulose/Filter Paper Sandwiches offer a convenient and reliable solution for Western blotting, nucleic acid detection, and hybridization techniques. Each sandwich is composed of a high-quality pure nitrocellulose membrane layered with thick absorbent blotting paper, pre-cut and pre-assembled to fit standard midi-size gel transfer systems.

These sandwich assemblies are designed for use in semi-dry and tank transfer systems, providing superior membrane contact and efficient buffer wicking, ensuring uniform transfer and high signal reproducibility.

Key Features

• Two Pore Sizes Available:
  • 0.2 μm (LC2009): Ideal for transferring small proteins and short nucleic acids
  • 0.45 μm (LC2006): Versatile option for proteins >20 kDa
• Pre-Assembled Format: Saves time and improves workflow consistency
• High Binding Capacity:
  • LC2009: ~80 µg/cm²
  • LC2006: Optimized for larger molecular targets
• Thick Blotting Paper (2.5 mm): Provides excellent buffer distribution for even transfer
• Hydrophobic and Electrostatic Binding Interactions: Stable retention of biomolecules
• Wide Detection Compatibility: Supports chemiluminescence, fluorescence, radiolabeling, and colorimetric methods

Specifications

Applications

• Western blotting for low molecular weight proteins
• Southern and Northern blotting of nucleic acids <300 bp
• Dot blot and slot blot assays
• Fluorescent and radiolabeled detection protocols
• High-sensitivity assays for low-abundance targets

Invitrogen™ Nitrocellulose/Filter Paper Sandwiches  provide a convenient, high-performance solution for the transfer of small biomolecules in blotting workflows. With optimized dimensions, high binding capacity, and excellent compatibility across detection platforms, they are an ideal choice for researchers performing semi-dry or vertical transfer blots requiring high sensitivity and reproducibility.