Invitrogen™ Lauria Broth Base (Miller's Lb Broth Base), Powder

Invitrogen™ Luria Broth Base (Miller's LB Broth Base) is a versatile, nutrient-rich medium commonly used for bacterial growth and maintenance. It supports rapid expansion and high-density cultures of Escherichia coli and is suitable for various molecular biology applications, including coliphage plaque assays.

Key Features:

1. Rich Medium:
   • Provides essential nutrients for robust bacterial growth and maintenance.
2. Miller’s Formulation:
   • Contains 10 g/L NaCl, optimized for general E. coli culture.
   • Ideal for non-salt-sensitive antibiotic selection.
3. Convenient Preparation:
   • Easy-to-use powder form that dissolves readily in water.
   • Autoclave sterilization ensures a contamination-free medium.
4. pH Stability:
   • Adjusted to pH 7.0 for optimal bacterial growth.

Applications:

1. Bacterial Culture:
   • Maintenance and rapid expansion of E. coli and other coliforms.
2. Molecular Biology:
   • Ideal for recombinant DNA cloning, plasmid preparation, and protein expression.
3. Coliphage Assays:
   • Supports phage plaque formation.

Specifications:

Catalog Numbers and Quantities:

Preparation Instructions:

1. Dissolve Powder:
   • Add 25 g of LB Broth Base powder to 1 L of distilled water.
2. Adjust pH:
   • Set pH to 7.0 using 5 N NaOH.
3. Sterilize:
   • Autoclave the medium for 25 minutes at 121°C.
4. Cool and Use:
   • Allow the medium to cool before use.

Comparison of LB Variants:

Advantages:

Handling and Storage:

• Storage Conditions: Store at room temperature to ensure long-term stability.
• Shelf Life: Check packaging for expiration date.

For high-quality and reliable bacterial growth, Invitrogen™ Luria Broth Base (Miller's LB Broth Base) is an excellent choice for research and molecular biology applications.