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Invitrogen™ IFN gamma Monoclonal Antibody (XMG1.2), APC, eBioscience™
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Rat Anti-Mouse IFN-γ | APC-Conjugated Monoclonal Antibody for Intracellular Flow Cytometry
The XMG1.2 clone is a well-characterized, rat IgG1 monoclonal antibody specific for mouse interferon-gamma (IFN-γ), a key type II cytokine involved in immune regulation, antiviral responses, and macrophage activation. This antibody is conjugated to allophycocyanin (APC) for detection by red laser (633–647 nm) in flow cytometry applications.
The antibody is widely used and cited in over 220 publications, making it a reliable tool for intracellular cytokine staining, especially in T cell and NK cell functional studies.
Key Features and Benefits
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Specific to Mouse IFN-γ
Reacts with mouse interferon-gamma, a 20 kDa cytokine produced by activated T cells, B cells, and NK cells. -
APC Conjugate
Excitation at 633–647 nm, emission at 660 nm, compatible with red laser channels in standard flow cytometers. -
Flow Cytometry-Validated
Optimized for intracellular staining; enables precise detection of IFN-γ production following stimulation. -
Neutralizing Capability
XMG1.2 is a neutralizing antibody, useful for functional blocking in immunological assays. -
High Citability and Validation
Extensively validated in literature and internal testing; verified via cell treatment-based expression profiling.
Applications
- Intracellular cytokine staining (ICS) by flow cytometry
- T cell and NK cell activation analysis
- Cytokine profiling in inflammation, infection, and immunotherapy
- Murine Th1 polarization assays
- Functional studies involving IFN-γ neutralization
Technical Specifications
| Property | Details |
|---|---|
| Clone | XMG1.2 |
| Host / Isotype | Rat / IgG1, kappa |
| Conjugate | Allophycocyanin (APC) |
| Excitation / Emission Max | 651 / 660 nm |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control, APC (eBRG1) |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.2 with 0.09% sodium azide |
| Storage Conditions | 4°C, protect from light, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| Filter Sterilization | 0.2 µm post-manufacturing filtered |
| RRID | AB_469504 |
| Species Reactivity | Mouse |
| Published Reactivity | Mouse, Human, Rat |
| Unit Sizes Available | 50 µg (Cat. No. 17-7311-81), 100 µg (Cat. No. 17-7311-82) |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Target Information – IFN-γ
Interferon gamma (IFN-γ) is a multifunctional cytokine involved in host defense, anti-tumor responses, and immunoregulation. Produced primarily by CD4+ Th1 cells, CD8+ cytotoxic T lymphocytes, and NK cells, IFN-γ drives macrophage activation, upregulates MHC I and II, and exhibits antiviral and antiproliferative properties. It functions through the JAK-STAT pathway and often synergizes with TNF-alpha and other pro-inflammatory cytokines. Dysregulation of IFN-γ has been implicated in various autoimmune and infectious diseases.
Invitrogen™ IFN gamma Monoclonal Antibody (XMG1.2), APC, eBioscience™ is a trusted, publication-validated reagent for detecting and quantifying IFN-γ in mouse models. Its strong specificity, neutralizing activity, and flow cytometry-optimized APC conjugation make it an essential tool for immunological research focused on Th1 responses, cytokine profiling, and cell-mediated immunity.
- Pack Size: 50 µg 100 µg