Invitrogen™ Fixation Medium (Medium A)

The Invitrogen™ Fixation Medium (Medium A) is a key component of the FIX & PERM™ Cell Permeabilization Kit, specifically designed for use in flow cytometry. This reagent fixes cells in suspension, preserving cell structure and enabling subsequent permeabilization for intracellular staining. It maintains scatter characteristics for accurate analysis while reducing background staining, making it an essential tool for studying intracellular targets alongside surface markers.

Key Features:

1. Efficient Cell Fixation:
   • Stabilizes cells for intracellular staining without compromising scatter characteristics.
2. Reduced Background:
   • Minimizes non-specific staining, enhancing signal clarity.
3. Simultaneous Staining:
   • Compatible with fluorochrome-labeled antibodies, allowing for intracellular and surface marker detection in a single workflow.
4. Flexible Application:
   • Works effectively with a variety of biological samples, including cell lines, whole blood, and bone marrow.
5. Component of FIX & PERM™ Kit:
   • Used in conjunction with Permeabilization Medium (Medium B) for complete fixation and permeabilization.

Specifications:

Applications:

1. Flow Cytometry:
   • Fixes cells in preparation for intracellular staining of cytokines, transcription factors, and other intracellular proteins.
2. Intracellular Cytokine Staining:
   • Facilitates detection of cytokines such as IFN-γ, IL-2, and IL-17A.
3. Multicolor Staining Panels:
   • Maintains cell morphology and scatter characteristics, enabling accurate gating in complex staining workflows.
4. Immunophenotyping:
   • Suitable for simultaneous detection of surface and intracellular markers.
5. Sample Types:
   • Validated for use with a variety of biological samples, including whole blood, bone marrow, and cultured cell lines.

Key Benefits:

1. Preserves Cell Morphology:
   • Maintains scatter characteristics for accurate flow cytometric analysis.
2. Minimizes Background:
   • Reduces non-specific staining, improving signal clarity for intracellular staining.
3. Simplifies Protocols:
   • Allows for simultaneous intracellular and surface marker staining, streamlining workflows.
4. Flexible and Robust:
   • Compatible with multiple sample types and fluorochrome-labeled antibodies.
5. Prepares Cells for Permeabilization:
   • Works in tandem with Permeabilization Medium (Medium B) for effective intracellular staining.

How It Works:

1. Fixation Step:
   • Incubate cells with Fixation Medium (Medium A) for 15 minutes at room temperature to stabilize intracellular targets.
2. Wash Step:
   • Wash cells to remove excess fixation medium.
3. Permeabilization and Staining:
   • Proceed with permeabilization using Permeabilization Medium (Medium B) and intracellular staining with fluorochrome-labeled antibodies.

Storage and Handling:

• Store at room temperature for long-term stability.
• Protect from extreme temperatures to maintain reagent efficacy.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.