Invitrogen™ eBioscience™ IFN gamma Monoclonal Antibody (XMG1.2), Brilliant Violet™ 711

The Invitrogen™ IFN gamma Monoclonal Antibody (XMG1.2), conjugated to Brilliant Violet™ 711 (BV711), is a premium reagent designed for intracellular detection of mouse IFN gamma using flow cytometry. IFN gamma, a key cytokine produced by activated T cells, NK cells, and B cells, plays a critical role in immune regulation, macrophage activation, and antiviral responses. This antibody is validated for intracellular staining, offering bright fluorescence for clear and reliable detection.

Key Features:

1. Target and Specificity:
   • Recognizes mouse IFN gamma, a cytokine involved in immune regulation, antiviral activity, and macrophage activation.
2. Clone and Conjugate:
   • Clone: XMG1.2, a neutralizing antibody known for high specificity and reliability.
   • Conjugate: Brilliant Violet™ 711, a tandem dye offering bright fluorescence with excitation at 407 nm and emission at 713 nm.
3. Validated Applications:
   • Optimized for intracellular staining in flow cytometry.
4. Bright and Stable Fluorescence:
   • BV711 provides high signal intensity, minimizing background and ensuring clear separation of positive and negative populations.
5. Pre-Titrated and Ready-to-Use:
   • Supplied at a concentration of 0.2 mg/mL, with recommended usage of 1 µg per test.

Specifications:

Applications:

1. Flow Cytometry:
   • Detect intracellular IFN gamma expression in mouse T cells, NK cells, and other immune cells.
   • Ideal for use in multicolor staining panels.
2. Th1 Research:
   • Study IFN gamma's role in Th1 differentiation and immune responses.
3. Macrophage Activation Studies:
   • Investigate IFN gamma's effects on macrophage activation and cytokine production.
4. Cytokine Profiling:
   • Analyze IFN gamma production in immune cells in response to antigen or mitogen stimulation.
5. Immunotherapy Research:
   • Explore IFN gamma's involvement in tumor immunity and therapeutic interventions.

Key Benefits:

1. Bright Fluorescence:
   • BV711 offers superior brightness and stability for clear identification of IFN gamma-positive cells in flow cytometry.
2. Minimal Background:
   • High specificity of the XMG1.2 clone ensures accurate results with minimal non-specific binding.
3. Reproducibility:
   • Pre-titrated and validated for consistent performance across experiments.
4. Multicolor Panel Compatibility:
   • BV711's spectral properties make it highly compatible with other fluorochromes in multicolor staining panels.
5. User-Friendly:
   • Ready-to-use liquid formulation simplifies experimental workflows.

Usage Recommendations:

1. Sample Preparation:
   • Stimulate mouse splenocytes with appropriate antigens or mitogens to induce IFN gamma production.
2. Staining Protocol:
   • Use the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) for optimal intracellular staining.
   • Add 1 µg of antibody per 100 µL of cell suspension.
3. Flow Cytometry:
   • Analyze samples using a flow cytometer equipped with a violet laser (407 nm).
4. Fixation:
   • Fix samples in IC Fixation Buffer to preserve fluorescence for up to 3 days.
5. Minimize Non-Specific Binding:
   • Use Brilliant Stain Buffer™ to reduce polymer dye interactions when combining multiple Brilliant Violet™ reagents.

Storage and Handling:

• Store at 4°C in the dark to protect the Brilliant Violet™ 711 fluorochrome.
• Avoid freezing, as freezing may degrade the antibody or fluorochrome.

Target Information:

• IFN gamma:
  • A dimeric cytokine (~45 kDa) secreted by activated T cells, NK cells, and B cells.
  • Plays a critical role in macrophage activation, MHC class I and II expression, and antiviral responses.
  • Acts via the JAK-STAT signaling pathway, synergizing with other cytokines to regulate immune responses.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.