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Specifications:
| Application | RNA Extraction | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Sample Prep | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ Dynabeads™ Oligo(dT)25 are magnetic beads specifically designed to isolate mRNA directly from crude cell lysates, tissue, plant samples, or total RNA without the need for a total RNA purification step. By selectively binding polyadenylated (poly(A)+) RNA molecules, these beads deliver highly pure, intact mRNA suitable for downstream molecular biology workflows, including RT-PCR, cDNA synthesis, and transcriptome profiling.
Unlike column-based or spin filter methods, Dynabeads™ use magnetic separation to minimize RNA loss and shear stress, offering fast, gentle, and efficient mRNA purification. This technology supports high-throughput workflows and enables direct on-bead cDNA synthesis, making it ideal for single-cell and low-input transcriptomics.
Key Features and Benefits
- Highly specific capture of poly(A)+ mRNA using oligo(dT)25-coated magnetic beads
- No ribosomal RNA or small RNA contamination – no DNase or rRNA subtraction required
- Rapid 15-minute protocol – no columns, no centrifugation, no clogging
- High binding capacity – yields up to 10 µg of mRNA per mL of beads
- Compatible with ultra-low input – suitable for single-cell to bulk samples
- Direct-to-cDNA synthesis – enzymatic reactions can be performed on beads
- Flexible elution – elute in volumes as low as 5 µL or skip elution for bead-bound workflows
How It Works
- Hybridization: Oligo(dT)25 on Dynabeads™ hybridizes with poly(A) tails of mRNA
- Magnetic separation: Beads (with bound mRNA) are rapidly pulled to the tube wall using a magnet
- Washing: Contaminants like rRNA, tRNA, DNA, and proteins are washed away
- Elution or direct use: Purified mRNA can be eluted or used directly for cDNA synthesis
Applications
- mRNA isolation from cells, tissues, plants, and total RNA
- cDNA synthesis, including first-strand synthesis
- RNA-Seq library prep and transcriptomics
- Gene cloning and RT-qPCR
- RACE, SAGE, primer extension, and subtractive hybridization
- Single-cell transcriptomics and low-input RNA workflows
Technical Specifications
| Attribute | Specification |
|---|---|
| Format | Magnetic beads with covalently bound Oligo(dT)25 |
| Binding Target | Poly(A)+ RNA (mRNA) |
| Final Product | Isolated mRNA |
| Purification Time | ~15 minutes |
| Yield | Up to 10 µg mRNA/mL of beads |
| Sample Compatibility | Cells (≤10⁶), Tissue (≤50 mg), Plant (≤100 mg), or total RNA (≤75 µg) |
| Elution Volume | 5–20 µL (or direct use on beads) |
| High-throughput Compatibility | Yes |
| Storage Conditions | 4 °C |
| Shipping Conditions | Room Temperature |
| Unit Sizes | 2 mL (Cat. No. 61002), 5 mL (Cat. No. 61005) |
Invitrogen™ Dynabeads™ Oligo(dT)25 offer a fast, column-free, and RNase-free solution for high-purity mRNA isolation directly from crude samples. With excellent specificity, flexibility, and low sample requirements, these beads are a gold standard in next-generation sequencing, transcriptomics, and molecular biology workflows.
- Pack Size: 5 mL 2 mL