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Invitrogen™ CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
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Specifications:
| Application | Flow Cytometry, Cell Analysis | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Bright, Stable Dye for Tracking Multiple Cell Divisions In Vitro and In Vivo
The CellTrace™ Violet Cell Proliferation Kit is a powerful tool for monitoring cell division history in vitro or in vivo using flow cytometry. This kit uses a violet-excited dye (405 nm excitation, 450 nm emission) that stably and covalently labels intracellular and surface amine groups, allowing tracking of up to 10+ cell generations via dye dilution. The resulting fluorescence is retained through multiple divisions, offering consistent, low-background signals for long-term studies.
Available in two pack sizes (20 assays or 180 assays), CellTrace™ Violet is widely used in immunology, oncology, stem cell research, and cell-based screening applications.
Key Features and Benefits
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Bright and Stable Fluorescence
Detects up to 10 or more generations with distinct, single-peak fluorescence intensity. -
Long-Term Signal Retention
Covalently binds to cellular proteins, ensuring stable signal over several days without impacting cell viability. -
Ideal for Multiparameter Flow Cytometry
Minimal spectral overlap with Alexa Fluor™ 488, FITC, PE, GFP, and mCherry for easy multiplexing. -
Ready-to-Use Single-Use Format
Includes dry dye and DMSO in convenient single-use vials for small-scale or large-scale experiments. -
Minimal Cytotoxicity
Dye labeling has low impact on cell proliferation or phenotype, making it ideal for functional studies.
Applications
- Cell proliferation and division tracking
- T cell activation and clonal expansion
- B cell or cancer cell proliferation assays
- Stem cell lineage and differentiation tracking
- In vivo cell tracking following adoptive transfer
- Cytotoxicity and drug response studies
Technical Specifications
| Property | Details |
|---|---|
| Product Name | CellTrace™ Violet Cell Proliferation Kit |
| Catalog Numbers | C34571 (20 Assays), C34557 (180 Assays) |
| Excitation / Emission | 405 / 450 nm |
| Dye Type | Violet-excited amine-reactive fluorescent dye |
| Detection Method | Fluorescence via dye dilution |
| Format | Solid (dry dye), with 100 µL DMSO included |
| Solubility | DMSO (dimethyl sulfoxide) |
| Reagent Type | CFSE & related compounds |
| Instrument Compatibility | Flow cytometer with 405 nm violet laser |
| Unit Size | Each (20 assays per kit) |
| Storage Conditions | –5°C to –30°C, protect from light |
| Shipping Conditions | Room temperature |
| Product Line | CellTrace™ |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Kit Contents
- 1 vial of CellTrace™ Violet dry dye
- 1 vial of DMSO (100 µL) for stock preparation
The CellTrace™ Violet Cell Proliferation Kit delivers bright, long-lasting, and well-retained fluorescent labeling for tracking cell divisions by flow cytometry. It is an essential tool for researchers studying immune response, cancer cell proliferation, stem cell fate, and cellular dynamics in vitro or in vivo.
- Pack Size: For 20 Assays For 180 Assays