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Invitrogen™ CD326 (EpCAM) Monoclonal Antibody (G8.8), FITC, eBioscience™
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Specifications:
| Application | immunocytochemistry, Flow Cytometry, Immunohistochemistry, Immunoprecipitation (IP), Affinity Purification (AP) | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ CD326 (EpCAM) Monoclonal Antibody (Clone G8.8), FITC-conjugated is a highly specific antibody designed for the detection of EpCAM (Epithelial Cell Adhesion Molecule, CD326), a widely expressed epithelial marker and pan-carcinoma antigen. This antibody is directly conjugated to FITC (Fluorescein Isothiocyanate), enabling rapid and sensitive fluorescence-based detection without the need for secondary antibodies.
Optimized for flow cytometry applications, this antibody provides reliable identification and analysis of epithelial cells and carcinoma-derived cells, making it a valuable tool in cancer research, cell biology, and immunophenotyping studies.
Key Features
- Specific for CD326 (EpCAM) epithelial marker
- Monoclonal antibody clone G8.8
- Directly conjugated with FITC fluorophore
- Excitation/Emission: 498 / 517 nm (compatible with blue laser systems)
- Reactivity: Mouse (validated; also reported in human studies)
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Optimized for:
- Flow Cytometry: ≤0.5 µg/test
- Supplied as liquid, ready-to-use antibody
- Purified by affinity chromatography
- Filtered through 0.2 µm for sterility and performance
Applications
This antibody is ideal for:
- Flow cytometric analysis of epithelial and tumor cells
- Immunophenotyping of epithelial-derived cell populations
- Detection of EpCAM-positive cancer cells
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Studies of:
- Tumor biology and carcinoma progression
- Cell adhesion and signaling pathways
- Cancer stem cells and circulating tumor cells (CTCs)
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Research applications in:
- Oncology
- Cell biology
- Immunology
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Invitrogen™ CD326 (EpCAM) Monoclonal Antibody (G8.8), FITC |
| Catalog Numbers | 11-5791-82 (100 µg), 11-5791-80 (25 µg) |
| Type | Antibody |
| Class | Monoclonal |
| Clone | G8.8 |
| Host / Isotype | Rat / IgG2a, κ |
| Species Reactivity | Mouse |
| Conjugate | FITC |
| Excitation / Emission | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Preservative | 0.09% sodium azide |
| Storage Conditions | 4°C, protect from light, do not freeze |
| Recommended Use | ≤0.5 µg/test (Flow Cytometry) |
| RRID | AB_11151709 |
Target Information
EpCAM (CD326) is a transmembrane glycoprotein (~40 kDa) expressed on most epithelial cells and highly overexpressed in many carcinomas, making it a key tumor-associated antigen. It mediates calcium-independent cell–cell adhesion and plays a role in regulating cell proliferation, differentiation, and signaling pathways.
EpCAM is widely used as a biomarker for:
- Epithelial-derived tumors
- Cancer stem cells
- Circulating tumor cell (CTC) detection
Its involvement in oncogenic signaling pathways (e.g., c-Myc, cyclins) makes it an important target in cancer diagnostics research and immunotherapy development.
Advantages
- Direct FITC conjugation for fast and simplified workflows
- High specificity for EpCAM-positive epithelial cells
- Ideal for flow cytometry and multiparametric analysis
- Strong performance with minimal background
- Reliable reagent for cancer and epithelial biology studies
The Invitrogen™ CD326 (EpCAM) Monoclonal Antibody (G8.8), FITC is a powerful tool for fluorescence-based detection of epithelial and carcinoma cells. Its high specificity, ease of use, and compatibility with flow cytometry platforms make it an essential reagent for oncology research, immunophenotyping, and cell analysis workflows.
- Pack Size: 25 µg 100 µg