Human Advanced Glycation End products (AGEs) ELISA Kit

High-Sensitivity Sandwich ELISA for Quantitative Detection of Human AGEs

The Human Advanced Glycation Endproducts (AGEs) ELISA Kit is a research-use-only sandwich ELISA system designed for the quantitative measurement of AGEs in human serum, plasma, culture media, urine, tissue extracts, and other biological fluids. Based on a pre-coated microplate with an anti-AGEs antibody, the assay allows for sensitive and specific detection of AGEs using horseradish peroxidase (HRP)-labeled detection and a TMB colorimetric substrate.

With a detection range of 26–1600 ng/mL, and a sensitivity as low as 5 ng/mL, this kit is ideal for academic, biomedical, and pharmaceutical research involving oxidative stress, diabetes complications, and inflammation pathways.

Key Features & Benefits

✅ High Sensitivity & Precision – Detection limit of 5 ng/mL, with intra-assay CV <10% and inter-assay CV <12%

✅ Wide Dynamic Range – 26 to 1600 ng/mL for quantifying AGEs across a broad spectrum

✅ Validated for Multiple Sample Types – Compatible with serum, plasma, urine, culture media, CSF, tissue lysates, and more

✅ Standardized Procedure – Pre-coated 96-well plate simplifies workflow and improves reproducibility

✅ Comprehensive Kit Format – Includes ready-to-use reagents, standards, buffers, and user manual

✅ Storage & Shelf Life – Store at 2–8°C; 6-month validity period from date of manufacture

Applications

• Biomedical Research – AGEs detection in diabetes, cardiovascular diseases, and neurodegeneration
• Cell Culture Studies – Quantifying secreted AGEs from treated or genetically modified cell lines
• Tissue & Organ Analysis – AGE level measurement in tissues under oxidative or metabolic stress
• Pharmaceutical Research – Evaluating the effect of AGE inhibitors or anti-inflammatory compounds
• Clinical Research – Non-diagnostic evaluation of AGE levels in patient-derived samples

Technical Specifications

Kit Contents

Sample Preparation

Compatible with:

• Serum and Plasma: Centrifuge at 2000–3000 rpm for 20 min
• Urine and CSF: Centrifuge and use supernatant
• Tissue Lysates: Homogenize in PBS, centrifuge, and collect supernatant
• Cell Culture Supernatants: Centrifuge and use clarified media
• Intracellular Components: Lyse with freeze/thaw cycles, centrifuge, and use supernatant

Assay Procedure (Simplified)

1. Dilute standards and samples according to instructions
2. Add 50 μL of standards or samples to wells + 50 μL HRP-conjugate reagent
3. Incubate at 37°C for 30 minutes
4. Wash plate 5 times
5. Add 50 μL each of Chromogen A & B, incubate 15 min in the dark
6. Add 50 μL stop solution
7. Read OD at 450 nm within 15 minutes

The Human AGEs ELISA Kit is a powerful, easy-to-use analytical tool for the quantitative detection of Advanced Glycation Endproducts in biological samples. Its high sensitivity, broad sample compatibility, and robust performance make it a preferred choice for researchers studying metabolic disorders, oxidative stress, and AGE-related pathologies.