Greiner CELLview™ Cell culture dishes, glass bottom

The CELLview™ Cell Culture Dish with a glass bottom and 4 compartments is a premium tool designed for advanced cell culture and high-resolution imaging applications. Manufactured with high-quality polystyrene (PS) and integrated borosilicate glass, this dish ensures superior optical performance and versatility for simultaneous multiplex analysis.

Product Features

1. Material and Construction:
   • Glass Bottom: Achromatic borosilicate glass, hydrolytic class 1 (DIN ISO 719), with a thickness of 175 µm ± 15 µm.
   • Plastic Body: Made from high-quality polystyrene (PS) for durability.
   • Surface Treatment: Tissue culture (TC) treated to enhance cell attachment and growth.
2. Sterility and Safety:
   • Free of detectable DNase, RNase, and human DNA.
   • Certified non-pyrogenic and non-cytotoxic.
   • Electron beam sterilized.
3. Optical Properties:
   • Maximal spectral transmission with no autofluorescence, ensuring compatibility with fluorescence and confocal microscopy.
   • Embedded glass bottom ensures maximal planarity for imaging consistency.
4. Design:
   • 4 Compartments: Each compartment allows for simultaneous experiments or drug treatments.
   • Growth area: 1.9 cm²/compartment.
   • Total volume: 1.5 mL/compartment.
   • Working volume: 0.1 mL for seeding or staining; 0.5 mL for cultivation.
5. Packaging:
   • Inner pack: 10 dishes per bag.
   • Packing unit: 40 dishes per case.
   • Packaging dimensions: 195 x 142 x 122 mm.
   • Packaging weight: 0.35 kg.

Applications

1. Live-Cell Imaging:
   • Ideal for time-lapse experiments and fluorescence microscopy to study live-cell dynamics, such as Golgi apparatus morphology or drug-induced changes.
2. Drug Screening:
   • Subdivided compartments enable the simultaneous testing of multiple drugs, reducing variability and enhancing comparative analyses.
3. Multiplex Analysis:
   • Four compartments allow parallel experiments with different conditions or treatments.
4. Advanced Microscopy:
   • Suitable for high-resolution techniques like confocal microscopy, fluorescence imaging, and phase-contrast microscopy.
5. Tissue Culture:
   • Optimized for culturing sensitive and adherent cell lines.

Advantages

1. Enhanced Optical Clarity:
   • The glass bottom provides clear, distortion-free visualization for microscopy.
2. Multiplex Analysis:
   • Four separate compartments save time and resources by allowing simultaneous testing.
3. Precision Design:
   • Embedded glass bottom ensures planarity, eliminating optical aberrations during imaging.
4. Versatile Usage:
   • Supports various experimental setups, including live-cell imaging, drug treatments, and cell culture.
5. Efficiency:
   • Compatible with small working volumes, minimizing reagent use for seeding, staining, and cultivation.

Detailed Use Case

Drug Treatment During Live-Cell Imaging:

• The dish supports the application of different drugs to separate compartments for real-time observation of cellular responses.
• Example Study: Golgi Apparatus Morphology:
  • Control: No drugs added, showing stable Golgi morphology over time.
  • Nocodazole: Induces Golgi fragmentation within 10-15 minutes, with visible effects after 3 hours.
  • Latrunculin B: Causes Golgi compaction, with changes beginning in 10-20 minutes and completing within an hour.
  • Brefeldin A: Rapidly redistributes the Golgi to the ER within 5 minutes.

Specifications

The CELLview™ Cell Culture Dish combines a high-quality glass bottom with a TC-treated polystyrene body, offering unparalleled performance for advanced microscopy and multiplex analysis. Its design is ideal for drug screening, live-cell imaging, and other cell culture applications requiring high optical clarity and precision.