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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ Viral Production Medium is a chemically defined, serum-free, and protein-free medium developed specifically for the growth and transfection of Viral Production Cells 2.0 (suspension-adapted HEK 293F-derived cells). As a critical component of the AAV-MAX Helper-Free AAV Production System, it enables efficient and scalable adeno-associated virus (AAV) production with consistently high titers.
This animal origin-free formulation supports viral production workflows from shake flask to bioreactor scale, delivering robust performance under ISO 13485 and FDA-registered manufacturing standards.
Key Features
- Optimized for Viral Production Cells 2.0 (HEK 293 suspension cells).
- Supports AAV production as part of the AAV-MAX Helper-Free AAV Production System.
- Enables high cell densities (>1.2 × 10⁷ cells/mL).
- Achieves AAV titers >5 × 10¹⁰ vg/mL (unpurified).
- Compatible with workflows from shake flask to large-scale bioreactor.
- Chemically defined, serum-free, protein-free, and animal origin-free.
- Requires supplementation with 4 mM GlutaMAX™ Supplement (Cat. No. 35050038) prior to use.
- Manufactured under ISO 13485, MDSAP, and FDA-registered facilities.
Specifications
| Parameter | Details |
|---|---|
| Cell Line | Viral Production Cells 2.0 |
| Cell Type | HEK 293 (suspension) |
| Expression System | Mammalian |
| Application | AAV Production |
| Format | Liquid, Bottle |
| Product Line | AAV-MAX |
| Product Type | AAV Production Medium |
| Classification | Serum-free, Protein-free, Animal origin-free, Chemically defined |
| Culture Type | Suspension cell culture |
| Additives | Glucose, Sodium Pyruvate (requires GlutaMAX supplement) |
| Without Additives | No GlutaMAX |
| pH | 7.0 |
| Sterility | Sterile-filtered |
| Shelf Life | 12 months from date of manufacture |
| Shipping Condition | Room temperature |
| Regulatory Status | RUO – Research Use Only |
| Manufacturing Quality | ISO 13485, MDSAP, FDA-registered |
| Unit Sizes | A4817901: 1 L |
Applications
- AAV production in HEK 293-derived Viral Production Cells 2.0.
- Scalable workflows from bench to bioreactor.
- Gene therapy research and viral vector development.
- High-titer virus production for preclinical research.
The Gibco™ Viral Production Medium is a specialized, high-performance medium tailored for AAV manufacturing workflows using Viral Production Cells 2.0. With its serum-free, animal origin-free, and chemically defined formulation, it ensures consistency, scalability, and regulatory reliability. Supporting densities beyond 1.2 × 10⁷ cells/mL and AAV titers exceeding 5 × 10¹⁰ vg/mL, this medium is the preferred choice for researchers developing viral vectors and advancing gene therapy innovations.
- Pack Size: 1L 6 x 1L 10 L bag