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Gibco™ RPMI 1640 Medium, L-glutamine, HEPES, Phenol Red
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Enhance the performance and reliability of your mammalian cell culture with Gibco™ RPMI 1640 Medium, formulated with HEPES, L-glutamine, and phenol red for consistent pH buffering and optimized growth conditions. Originally developed by the Roswell Park Memorial Institute for human leukemic cells, this medium supports a broad range of suspension and adherent mammalian cell lines including HeLa, Jurkat, PBMC, and astrocytes.
Key Features
- Complete Modified Formula: Contains HEPES for stable pH buffering, L-glutamine for amino acid supplementation, and phenol red as a pH indicator.
- Wide Cell Line Compatibility: Suitable for culturing HeLa, Jurkat, MCF-7, PC12, PBMCs, astrocytes, and carcinoma cell lines.
- Ready-to-Use Liquid Format: Convenient sterile-filtered 500 mL bottle for direct use in lab workflows.
- High Nutrient Composition: Includes unique components like glutathione, biotin, vitamin B12, PABA, inositol, and choline for enhanced cellular function.
- Animal-Origin Free: Formulated without animal-origin components for reduced variability and improved reproducibility.
Applications
- Suspension and adherent mammalian cell culture
- Leukemic cell maintenance and propagation
- Oncology research involving carcinoma and transformed cell lines
- Immunological studies using PBMCs and Jurkat cells
- Neuroscience and astrocyte-related research
Specifications
| Attribute | Description |
|---|---|
| Product Name | RPMI 1640 Medium with HEPES |
| Form | Liquid |
| With Additives | L-glutamine, HEPES, Phenol Red |
| Without Additives | No Sodium Pyruvate |
| Cell Line Compatibility | HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes |
| Buffering System | Sodium Bicarbonate (2.0 g/L) + HEPES |
| pH Conditions | 5–10% CO₂ required |
| Serum Requirement | Requires supplementation, e.g., 10% FBS |
| Sterility | Sterile-filtered |
| Storage Conditions | 2–8°C; protect from light |
| Shipping Conditions | Ambient |
| Shelf Life | 12 months from date of manufacture |
| Manufacturing Standard | cGMP-compliant; ISO 13485 |
| Animal Origin | Animal-origin free |
Comparison Table with Related Media
| Medium | Buffering System | Key Supplements | Application Focus |
|---|---|---|---|
| RPMI 1640 with HEPES | Sodium Bicarbonate + HEPES | Glutamine, Phenol Red | Broad mammalian cell culture |
| DMEM (high glucose) | Sodium Bicarbonate | Glucose, Amino Acids | Fast-growing adherent cells (e.g. fibroblasts) |
| MEM | Sodium Bicarbonate | Minimal nutrients | Primary and non-transformed cells |
Unique Advantages
- Stable pH Control: HEPES buffering minimizes pH shifts during handling.
- Nutrient-Dense Composition: Unique inclusion of PABA, inositol, and glutathione.
- Validated for Research Use: Trusted across oncology, immunology, and neuroscience laboratories.
- Flexible Storage & Handling: Ships at ambient temperature; stores refrigerated.
- Pack Size: 1L 100 mL 500 mL 10 x 500 mL 6 x 1L
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