Gibco™ Recombinant Human Laminin 521 (rhLaminin-521)

Gibco™ Recombinant Human Laminin 521 (rhLaminin-521) is a xeno-free, hESC-qualified extracellular matrix protein designed to provide a defined, physiological surface for feeder-free culture of human pluripotent stem cells (PSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs).

When used in conjunction with Essential 8™ Medium, rhLaminin-521 promotes robust cell survival under stress, supports >20 passages without karyotypic abnormalities, and maintains full differentiation potential into all three germ layers. It also improves reprogramming efficiency from human somatic cells and supports feeder-dependent to feeder-free transitions without the need for ROCK inhibitors.

Key Features

• Feeder-Free PSC Culture – Supports long-term, stable expansion without karyotypic abnormalities.
• High Reprogramming Efficiency – Enhances colony formation during somatic cell reprogramming.
• Physiologically Relevant – Mimics the native human stem cell niche; expressed in blastocysts.
• Xeno-Free – Suitable for defined, animal-origin-free culture systems.
• Scalable Culture – Enables single-cell passaging with stable phenotype maintenance.

Applications

• Feeder-free culture of hESCs and hiPSCs
• Reprogramming of human fibroblasts and other somatic cells
• Disease modeling and in vitro differentiation studies
• Stem cell scale-up and bioprocessing
• Transition from feeder-dependent to feeder-free systems

Technical Specifications

Gibco™ rhLaminin-521 provides a reliable, high-performance, xeno-free substrate for pluripotent stem cell research. Its ability to support robust expansion, efficient reprogramming, and genetic stability makes it an ideal choice for advanced stem cell culture applications in both research and translational workflows.