Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) (50 mg/mL)

Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) is a widely used aminoglycoside antibiotic designed for selecting and maintaining genetically modified cells. Derived from the bacterium Micromonospora rhodorangea, G418 Sulfate functions by irreversibly binding to ribosomal subunits, disrupting protein synthesis, and ultimately inhibiting cell growth.

This antibiotic is commonly used for stable cell line selection, ensuring only cells expressing G418 resistance genes (APH (3') – I and APH (3') – II from E. coli) survive. Its broad applicability in both prokaryotic and eukaryotic cells makes it an essential reagent in molecular biology, genetic engineering, and recombinant protein production.

Key Features & Benefits

✅ Highly Effective Selection Agent – Ensures stable transfection by eliminating non-resistant cells

✅ Defined Working Concentration – 100–200 µg/mL for bacteria; 200–500 µg/mL for mammalian cells

✅ Ready-to-Use Liquid Formulation – Provided as a sterile-filtered, 50 mg/mL solution in water

✅ Broad Application – Suitable for mammalian, insect, and bacterial cell culture

✅ High Purity & Consistency – Manufactured under ISO 13485 and cGMP guidelines

✅ Dual-Site Production – Ensures supply chain continuity from Grand Island, NY (USA) & Scotland, UK

Applications

🔬 Stable Cell Line Generation – Commonly used in genetic engineering to select for transfected cells carrying a resistance gene

🦠 Prokaryotic & Eukaryotic Selection – Efficient for bacterial and mammalian cell culture

🧬 Gene Expression Studies – Supports studies involving genetic modifications and recombinant protein production

🛠️ Biotechnology & Pharmaceutical Research – Used in research applications requiring selective antibiotic screening

Quality & Manufacturing Standards

Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) is manufactured under strict quality control protocols to ensure consistent performance across research applications.

cGMP-Compliant & ISO 13485 Certified

• Manufactured at two independent sites: Grand Island, NY (USA) and Scotland, UK
• ISO 13485 certified to meet global regulatory and quality standards
• Ensures batch-to-batch consistency and reliable performance
• Reduces supply chain risks by dual-site production

Product Specifications

Usage Guidelines

• Recommended Working Concentrations:
  • Bacteria: 100–200 µg/mL
  • Mammalian Cells: 200–500 µg/mL (varies based on cell type and sensitivity)
• Preparation & Handling:
  • Do not autoclave – use sterile-filtered solutions for dilution if needed
  • Store at 2–8°C to maintain product stability
  • Protect from contamination and prolonged exposure to ambient temperatures

Ordering Information

Shelf Life: 24 months from the date of manufacture.

Regulatory & Safety Information

⚠️ For Research Use Only. Not for use in diagnostic or therapeutic procedures.

• Always handle under appropriate biosafety conditions.
• Follow laboratory protocols for safe disposal and storage.
• Refer to the Safety Data Sheet (SDS) for detailed hazard and handling information.

Why Choose Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate)?

🔹 Proven Performance – Trusted by researchers worldwide for stable cell line selection

🔹 Dual-Site cGMP Manufacturing – Ensures product availability and reliability

🔹 High Purity & Consistency – Manufactured under strict quality control protocols

🔹 Ready-to-Use Formulation – No additional preparation required

For bulk orders, technical support, or additional information, contact LabMart Limited today.