GeneJET™ Plasmid Midiprep Kit

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

• Efficient—high yields up to 200 μg of high quality plasmid DNA from 50 mL of bacterial culture

• High concentration of DNA purified— >400 ng/μL

• Convenient—spin column format, centrifuge or vacuum protocols

• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

• FastDigest restriction enzyme digestion

• Automated fluorescent sequencing

• PCR

• Cloning

• Transformation

• In vitro transcription

• Transfection of robust cell lines