Agilent 4150 TapeStation System

The Agilent 4150 TapeStation System is an economic, entry-level automated electrophoresis platform for fast and reliable analysis of up to 16 DNA and RNA samples per run. Utilizing proven ScreenTape technology, this compact system provides highly reproducible quality control (QC) analysis for next-generation sequencing (NGS), microarray, and qPCR workflows.

Designed for low-throughput laboratories, the 4150 TapeStation offers superior ease-of-use, constant per-sample costs, and the ability to quickly switch between DNA and RNA ScreenTape assays. The system enables efficient nucleic acid quality assessment with RNA Integrity Number equivalent (RINe) and DNA Integrity Number (DIN) measurements, ensuring precise sample integrity evaluation.

Key Features & Benefits

1. Fast & Reliable Nucleic Acid QC
   • Delivers results in as little as 1–2 minutes per sample.
   • Processes 16 samples in under 20 minutes, reducing workflow bottlenecks.
2. Easy-to-Use ScreenTape Technology
   • Eliminates gel preparation, sample handling errors, and contamination risks.
   • Provides consistent, automated sample analysis with high reproducibility.
3. Flexible DNA & RNA Assay Options
   • Compatible with genomic DNA, cell-free DNA, total RNA, and mRNA assays.
   • Easily switch between DNA and RNA ScreenTape assays without recalibration.
4. Compact & Scalable Design
   • Small footprint (254 mm x 510 mm x 417 mm) saves bench space.
   • Allows seamless upgrade to the Agilent 4200 TapeStation for increased throughput.
5. Cost-Effective & Reproducible Results
   • Constant per-sample cost ensures predictable budgeting for QC workflows.
   • Standardized RINe and DIN scoring system for RNA/DNA quality assessment.

Product Specifications

Use Cases & Applications

1. Next-Generation Sequencing (NGS) Sample QC

• Ensures High-Quality DNA/RNA for NGS Library Preparation
  • Measures RNA integrity (RINe) for accurate RNA-Seq profiling.
  • Assesses DNA fragmentation and DIN values for whole-genome sequencing.
• Automated Electrophoresis for NGS Pipelines
  • Provides reproducible QC data for sample selection and library validation.
  • Reduces variability and manual errors in nucleic acid analysis workflows.

2. Genomic & Cell-Free DNA Analysis

• Genomic DNA Integrity Evaluation
  • Measures DNA Integrity Number (DIN) to assess DNA degradation levels.
  • Ideal for long-read sequencing and array-based genomic studies.
• cfDNA Analysis for Liquid Biopsy & Cancer Research
  • Enables quality control of circulating cell-free DNA (cfDNA) from plasma.
  • Supports fragment size analysis for biomarker discovery studies.

3. RNA Quality Control for Gene Expression & Transcriptomics

• Determines RNA Integrity for qPCR & Microarray Studies
  • Rapidly assesses total RNA and mRNA quality for transcriptomics research.
  • Supports biomarker discovery and gene expression analysis.
• RNA Sample Integrity for Stem Cell & Single-Cell Studies
  • Ensures high-quality RNA for single-cell sequencing applications.
  • Minimizes artifacts caused by RNA degradation.

4. Clinical Research & Biopharmaceutical Development

• Reliable Nucleic Acid QC for Drug Discovery & Cell Therapy Research
  • Facilitates standardized RNA/DNA analysis in regulated environments.
  • Supports assay development in molecular diagnostics.
• Essential for CRISPR & Genome Editing Workflows
  • Enables precise DNA quality assessment for gene-editing efficiency.
  • Ensures accurate genomic DNA integrity in CRISPR-based studies.

Instructions for Use

1. Load Samples:
   • Place 1 to 16 samples in the instrument tray.
   • Insert the appropriate DNA or RNA ScreenTape cartridge.
2. Run the TapeStation Analysis:
   • Start the automated electrophoresis process via software.
   • Samples are analyzed in 1–2 minutes per sample.
3. Interpret Data & Export Results:
   • View RINe or DIN values for RNA/DNA integrity assessment.
   • Export results in PDF, CSV, or LIMS-compatible formats.

Storage & Handling Instructions

• Storage Conditions:
  • Store the instrument in a temperature-controlled laboratory.
  • Keep ScreenTape reagents at recommended conditions (4°C or RT, depending on assay type).
• Handling Precautions:
  • Follow standard lab safety protocols when handling nucleic acid samples.
  • Ensure proper calibration and maintenance for reproducible results.
• Disposal Guidelines:
  • Dispose of used ScreenTape cartridges and reagents as per biosafety waste regulations.

Why Choose the Agilent 4150 TapeStation System?

✅ Fast & automated DNA/RNA integrity analysis in under 20 minutes

✅ Low-throughput system ideal for research & routine QC

✅ Proven ScreenTape technology for reproducible electrophoresis

✅ Seamless upgrade to the 4200 TapeStation for higher throughput

✅ Compact design saves valuable bench space

The Agilent 4150 TapeStation System is an affordable, entry-level electrophoresis platform designed for rapid, accurate nucleic acid QC, ensuring high-quality sample preparation for NGS, qPCR, and microarray workflows.