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Thermo Scientific™ EheI (SfoI) (10 U/μL), 500 units
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ EheI (SfoI) is a high-quality conventional restriction enzyme that recognizes the sequence 5′ GGC^GCC 3′ / 3′ CCG^CGG 5′, producing blunt ends. Optimized to function at 37 °C in Tango buffer, it provides reliable and reproducible cleavage for molecular cloning and DNA analysis workflows.
As part of Thermo Scientific’s portfolio of conventional restriction enzymes, EheI (SfoI) is validated within the Five Buffer System, ensuring maximum activity under standardized conditions. The inclusion of universal Tango buffer facilitates double digestions, while premixed BSA in all buffers stabilizes enzyme activity and sequesters contaminants for consistent performance.
Key Features
- High fidelity – complete digestion at 37 °C in Tango buffer.
- Superior quality – manufactured under stringent quality control standards.
- Five Buffer System – convenient, color-coded buffer system ensures optimal activity.
- Universal Tango buffer – enables efficient double digestions.
- Stabilized buffers – premixed BSA enhances enzyme stability and reliability.
- Wide compatibility – broad selection of isoschizomers available (BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping and SNP analysis.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | EheI (SfoI) |
| Recognition Sequence | 5′ GGC^GCC 3′ / 3′ CCG^CGG 5′ |
| Cleavage Product | Blunt ends |
| Isoschizomers | BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI |
| Optimal Reaction Temperature | 37 °C |
| Compatible Buffer | 10X Tango Buffer |
| Concentration | 10 U/µL |
| Quantity | 500 units |
| Methylation Sensitivity | CpG methylation-sensitive; not Dam/Dcm-sensitive |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Format
| Catalog Number | Quantity | Concentration | Format |
|---|---|---|---|
| ER0441 | 500 units | 10 U/µL | Enzyme + Tango buffer |
Thermo Scientific™ EheI (SfoI) (10 U/µL) offers precision and reliability for blunt-end DNA cleavage in cloning and genotyping workflows. Optimized for use with Tango buffer, it ensures efficient digestion while minimizing star activity. With stringent manufacturing standards, universal buffer compatibility, and proven performance, EheI (SfoI) is a trusted enzyme for molecular biology laboratories conducting high-quality DNA analysis and cloning experiments.