Invitrogen™ ProcartaPlex Human Basic Kit

ProcartaPlex™ Basic and Setup Kits Overview

ProcartaPlex™ Basic and Setup Kits provide the essential reagents and materials required for multiplex immunoassays based on Luminex™ xMAP technology. These kits are designed to enable the detection and quantitation of multiple protein biomarkers, including cytokines, chemokines, and growth factors, in various biological samples such as cell culture supernatants, serum, and plasma.

Key Features

1. Multiplex Protein Quantitation:
   • Enables simultaneous detection of multiple secreted proteins from a single sample.
2. Luminex™ xMAP Technology:
   • Utilizes bead-based multi-analyte profiling for robust and sensitive protein detection.
3. Pre-Configured Panels and Customizability:
   • Broad portfolio of pre-configured panels and combinable simplex kits.
   • Note: For combining Simplex Kits, an additional Basic Kit is required.
4. High Sensitivity:
   • Quantifies low-abundance proteins with assay sensitivity as detailed in the Certificate of Analysis.
5. Validated Reagents:
   • Manufactured to stringent standards akin to Platinum and Instant ELISA kits.
6. Flexible Sample Types and Volume:
   • Supports multiple sample types:
     • Serum/Plasma: 25 μL.
     • Cell Culture Supernatant: 50 μL.

Specifications

Kit Contents

The ProcartaPlex™ Basic Kit includes:

• Assay diluent.
• Wash buffers.
• SA-PE (Streptavidin-Phycoerythrin).
• Plates (does not include a Multiscreen Filter Plate).

Applications

1. Biomarker Profiling:
   • Quantify cytokines, chemokines, growth factors, and other protein biomarkers.
2. Multiplex Immunoassays:
   • Detect and quantitate multiple analytes in the same sample to conserve time and resources.
3. Clinical Research:
   • Ideal for studying inflammation, immune responses, and disease pathways in human samples.
4. Custom Panels:
   • Combine simplex kits to create custom panels tailored to specific research needs.

Advantages

Sample Types and Requirements

For Research Use Only

This kit is intended for research use only and is not suitable for diagnostic or clinical procedures.

Compatible Equipment

• Luminex™ instruments such as:
  • MAGPIX
  • FLEXMAP 3D
  • Luminex 200

Notes

• Basic Kits are mandatory for running combinable Simplex Kits.
• The Basic Kit does not include a Multiscreen Filter Plate; additional materials may be required depending on the assay setup.

ProcartaPlex™ Basic and Setup Kits provide a versatile and high-throughput solution for biomarker research, allowing precise quantitation of multiple analytes from minimal sample volumes in a single assay run.