Invitrogen™ FABP2 (intestinal) Human ELISA Kit

The Invitrogen™ FABP2 (Intestinal) Human ELISA Kit is designed for the quantitative detection of human FABP2 (Fatty Acid-Binding Protein 2) in serum, plasma, or supernatant samples. This sandwich ELISA is optimized for high specificity and sensitivity, with an analytical sensitivity of 25 pg/mL and an assay range from 0.024 to 100 ng/mL.

Key Features and Benefits

1. High Sensitivity and Specificity:
   • Analytical sensitivity of 25 pg/mL.
   • Assay range spanning from 0.024 to 100 ng/mL, allowing detection of low and high concentrations of FABP2.
2. Broad Sample Compatibility:
   • Compatible with human plasma, serum, and supernatants.
   • Requires only 50 µL of plasma or serum and 100 µL of supernatant per test.
3. Rigorous Validation:
   • Each kit batch is validated for sensitivity, precision (intra-assay CV <10%, inter-assay CV <12%), and lot-to-lot consistency.
4. Ease of Use:
   • Pre-coated 96-well microplate with a matched antibody pair ensures reliable results.
   • Total hands-on time of 1 hour 20 minutes and a total assay time of approximately 4 hours 45 minutes.
5. Quantitative and Reproducible:
   • Employs a colorimetric detection method compatible with standard microplate readers.
   • Offers reproducible results due to its robust design and validated reagents.
6. Comprehensive Kit Contents:
   • Includes all necessary components such as standards, diluents, detection antibodies, SAV-HRP, wash buffers, chromogen, stop solution, adhesive plate covers, and a pre-coated plate.

Kit Applications

The FABP2 Human ELISA Kit is ideal for:

• Biomedical Research:
  • Understanding the role of FABP2 in fatty acid transport and compartmentalization.
  • Studying FABP2 as a biomarker for intestinal health and diseases.
• Disease Studies:
  • Exploring its implications in metabolic disorders like obesity and diabetes.
  • Monitoring intestinal damage or permeability in various clinical conditions.
• Pharmacological Research:
  • Evaluating the impact of therapeutic agents on intestinal fatty acid metabolism.
  • Investigating the molecular mechanisms of intestinal fatty acid-binding proteins.

Technical Specifications

Target Information

FABP2 is a member of the fatty acid-binding protein family and is primarily expressed in intestinal epithelial cells. It plays a crucial role in:

• Fatty Acid Transport: Facilitates intracellular transport of long-chain fatty acids and lipophilic molecules.
• Metabolic Regulation: Involved in lipid metabolism and energy homeostasis.
• Biomarker Potential: Associated with intestinal health, integrity, and diseases like irritable bowel syndrome and Crohn's disease.

Kit Components

• Pre-coated 96-Well Plate: Simplifies the setup process.
• Standards and Diluent Concentrates: For generating a standard curve.
• Biotinylated Detection Antibody and SAV-HRP: Ensure precise and sensitive detection.
• Wash Buffer and TMB Substrate: Provide clean background and optimal signal-to-noise ratio.
• Stop Solution: For halting the colorimetric reaction.
• Adhesive Plate Covers: Prevent contamination and evaporation during the assay.

Gene and Protein Information

• Gene Symbol: FABP2
• Aliases: I-FABP, Intestinal-Type Fatty Acid-Binding Protein
• UniProt ID: P12104
• Role: Integral in lipid metabolism and cellular signaling pathways.

Storage and Stability

• Store the kit at 2–8°C.
• Shipping is maintained on wet ice to preserve reagent integrity.

The Invitrogen™ FABP2 Human ELISA Kit is a highly efficient, sensitive, and specific tool for detecting and quantifying FABP2. Its applications span basic research, disease studies, and therapeutic evaluations, making it an essential addition to any molecular biology or clinical research lab focusing on intestinal health and metabolism.