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Invitrogen™ DDAO galactoside (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside)
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Specifications:
| Application | Cell Analysis | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Molecular Biology Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₁₅H₁₁Cl₂NO₂ |
Fluorescent β-galactosidase substrate with large Stokes shift for sensitive enzyme activity detection
Invitrogen™ DDAO galactoside is a highly sensitive β-galactosidase substrate that produces a fluorescent hydrolysis product detectable in the far-red spectrum. Upon cleavage by β-galactosidase, DDAO galactoside yields a hydrolysis product with excitation/emission maxima of ~645/660 nm, making it ideal for use with 633 nm laser excitation. The substrate itself is also fluorescent (excitation/emission ~460/610 nm), and the ~200 nm spectral shift between the substrate and product allows for clear spectral separation during detection.
This property enables simultaneous monitoring of substrate and product, reducing interference and enhancing sensitivity in multi-parameter fluorescence assays. DDAO galactoside is particularly suited for flow cytometry, fluorescence microscopy, and reporter gene assays where β-galactosidase is used as a marker enzyme.
Key Features
- Large Stokes Shift: Distinct spectral separation (~200 nm) between substrate and product
- Far-Red Detection: Product excitable with 633 nm laser; emission ~660 nm
- High Sensitivity: Suitable for low-level β-galactosidase activity detection
- Versatile Applications: Compatible with flow cytometry, microscopy, and plate-based assays
- Stable Stock Solutions: Dissolve up to 5 mg/mL in anhydrous DMSO for long-term storage
Applications
- β-galactosidase reporter assays
- Flow cytometry-based enzyme activity detection
- Live-cell and fixed-cell fluorescence microscopy
- Enzyme kinetics and screening assays
- Multiplex fluorescence detection with minimal spectral overlap
Specifications
| Parameter | Value |
|---|---|
| Catalog Number | D6488 (also known as D-6488) |
| Chemical Name | 9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside |
| Quantity | 5 mg |
| Molecular Weight | 470.31 g/mol |
| Substrate Type | β-galactosidase substrate |
| Excitation/Emission (Substrate) | ~465/608 nm |
| Excitation/Emission (Product) | ~645/660 nm |
| Form | Solid |
| Solubility | Anhydrous DMSO |
| Storage Conditions | –5°C to –30°C, protect from light, desiccated |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
Invitrogen™ DDAO galactoside is a premier choice for researchers needing sensitive, far-red β-galactosidase detection with minimal background interference. Its large Stokes shift and compatibility with multiple fluorescence platforms make it highly suitable for multiplexed assays and live-cell enzymatic studies.