Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated), 500g

The Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base is a selective microbiological culture medium designed for the isolation and enumeration of yeasts and xerophilic molds from dried and low-moisture food products. This ISO-compliant medium is pre-supplemented with chloramphenicol, providing effective inhibition of bacterial growth while promoting selective fungal recovery.

Formulated according to BS ISO 21527-2:2008, this medium is ideal for microbiological quality control of dried foods, ensuring accurate enumeration and reliable detection of spoilage fungi.

Key Features

• Selective medium for enumeration of yeasts and xerophilic molds
• Pre-supplemented with chloramphenicol for bacterial inhibition
• Fully compliant with BS ISO 21527-2:2008 standards
• Contains dichloran to restrict fungal colony spreading
• Low water activity formulation improves recovery of xerophilic fungi
• Ready-to-use dehydrated powder reduces chemical handling
• Long shelf life of up to 5 years

Principle of Operation

This DG18 (ISO) medium contains:

• Dichloran, which restricts spreading of fast-growing fungi and allows clear colony separation
• Glycerol (18% w/w), which reduces water activity (aW) to approximately 0.95, promoting growth of xerophilic molds
• Chloramphenicol, which suppresses bacterial growth and improves fungal selectivity

This combination allows accurate detection and enumeration of yeasts and molds in dried and semi-dried food samples.

Standards Compliance

This culture medium is formulated in accordance with:

• BS ISO 21527-2:2008
• Microbiology of food and animal feeding stuffs
• Colony counting technique for products with water activity ≤0.95

This ensures reliable results and compliance with international food microbiology testing standards.

Applications

The pre-supplemented DG18 (ISO) Agar Base is widely used for:

• Enumeration of yeasts and molds in dried food products
• Food microbiology quality control testing
• Detection of fungal spoilage organisms
• Microbiological analysis of low-moisture foods

Typical sample types include:

• Spices and herbs
• Dried fruits
• Cereals and grains
• Nuts and seeds
• Confectionery products
• Dried meat and fish

Technical Specifications

Advantages

• Pre-supplemented with antibiotic to simplify preparation
• Improved selectivity by suppressing bacterial growth
• ISO-compliant for food microbiology testing
• Enhanced recovery of xerophilic fungi
• Accurate colony enumeration and identification
• Reduced laboratory preparation time and improved safety

The Thermo Scientific™ Oxoid™ Pre-supplemented DG18 (ISO) Agar Base (CM1151B) is a high-quality, ISO-compliant culture medium designed for reliable isolation and enumeration of yeasts and xerophilic molds in dried and low-moisture food products. Its pre-supplemented formulation with chloramphenicol ensures improved selectivity, making it an essential medium for food safety laboratories, quality control programs, and microbiological research.