Thermo Scientific™ Brilliance™ E. coli/Coliform Selective Medium (Dehydrated)

The Thermo Scientific™ Oxoid™ Brilliance™ E. coli/Coliform Selective Medium (Dehydrated) is a highly selective and chromogenic agar base designed for the detection and enumeration of Escherichia coli and other coliforms in food and water samples. Using two chromogens, Rose-Gal and X-Glu, this medium enables clear differentiation between E. coli (purple colonies) and other coliforms (pink colonies), making it an invaluable tool for food safety and environmental hygiene testing.

Key Features

1. Selective and Specific:
   • Incorporates sodium lauryl sulphate to inhibit Gram-positive organisms, ensuring selective growth of E. coli and coliforms.
2. Chromogenic Differentiation:
   • E. coli cleaves both chromogens (Rose-Gal and X-Glu), producing purple colonies.
   • Other coliforms cleave only Rose-Gal, resulting in pink colonies.
3. Indole Test Integration:
   • Contains tryptophan for rapid confirmation of E. coli via a spot indole test.
4. Easy-to-Read Results:
   • Clear differentiation between E. coli and other coliforms enhances diagnostic accuracy.
5. Wide Application Range:
   • Suitable for testing food, water, and environmental samples for hygiene monitoring.

Applications

1. Food Microbiology

• Detection and enumeration of E. coli and coliforms in food products, including dairy, meat, and processed foods.
• Ensures food safety and regulatory compliance.

2. Water Quality Testing

• Monitors E. coli and coliform contamination in drinking water, recreational water, and wastewater.

3. Environmental Microbiology

• Identifies E. coli and coliforms in soil, industrial effluents, and other environmental samples.

4. Public Health and Regulatory Testing

• Evaluates food and environmental hygiene as indicators of fecal contamination.

Specifications

Preparation Instructions

1. Suspend: Suspend 38.6g of Brilliance™ E. coli/Coliform Selective Medium in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation to dissolve completely. Do not autoclave.
3. Cool and Pour: Cool to 45–50°C, pour into sterile Petri dishes, and allow the medium to solidify.
4. Inoculate: Add the test sample and incubate as required.

Advantages

1. Selective and Chromogenic: Differentiates E. coli and coliforms clearly, even in mixed samples.
2. Enhanced Accuracy: Reduces interference from Gram-positive organisms via selective inhibition.
3. Rapid Confirmation: Integrated tryptophan enables quick indole test for E. coli.
4. Wide Utility: Applicable to food safety, water testing, and environmental monitoring.
5. Easy Interpretation: Chromogenic differentiation ensures quick and accurate results.

Diagnostic Features

Colony Appearance

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: Refer to the product label for expiration date.

Packaging

• Product: Brilliance™ E. coli/Coliform Selective Medium (Dehydrated)
• Quantity: 500g
• Yield: Sufficient for preparing approximately 12.9L of medium.

The Thermo Scientific™ Oxoid™ Brilliance™ E. coli/Coliform Selective Medium (Dehydrated) is an advanced, chromogenic agar designed for the selective detection and differentiation of E. coli and coliforms in food, water, and environmental samples. With its selective inhibition of Gram-positive organisms, chromogenic differentiation, and integrated indole test capability, this medium is essential for food safety and environmental monitoring. Its ease of use and accurate results make it an invaluable tool for microbiological laboratories.