Canine Ehrlichiosis Elisa Kit

The Canine Ehrlichiosis ELISA Kit is a highly sensitive and specific diagnostic tool designed for the detection of antibodies or antigens associated with Ehrlichia canis, the causative agent of canine ehrlichiosis. This tick-borne disease, transmitted by the brown dog tick (Rhipicephalus sanguineus), affects the immune system and blood cells in dogs. The kit uses a Sandwich-ELISA method to provide reliable results, making it suitable for clinical diagnostics and veterinary research.

Purpose

• Detection of Canine Ehrlichiosis:
  • Identifies the presence of Ehrlichia canis antigens or antibodies in canine serum or plasma.
• Applications:
  • Diagnosis of acute, subclinical, and chronic stages of canine ehrlichiosis.
  • Monitoring disease progression and response to treatment.
  • Screening for Ehrlichia infections in veterinary clinics or research studies.

Principle

The Canine Ehrlichiosis ELISA Kit utilizes the Sandwich-ELISA technique for high sensitivity and specificity. Below is the step-by-step mechanism:

1. Pre-Coated Microelisa Plate:
   • Wells are pre-coated with a specific antibody for Ehrlichia canis antigens or antibodies.
2. Sample and Standard Addition:
   • Serum or plasma samples, along with controls, are added to the wells, allowing antigens or antibodies to bind to the pre-coated specific antibodies.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated detection antibody specific to Ehrlichia is added, forming a complex with the bound target.
4. Washing Step:
   • Unbound components are washed away to minimize background noise.
5. Substrate Reaction:
   • A TMB substrate solution is added, which reacts with the HRP enzyme, producing a blue color in positive wells.
6. Stop Solution:
   • The reaction is stopped, changing the color to yellow.
7. Optical Density Measurement:
   • The optical density (OD) is measured at 450 nm using a spectrophotometer. The OD value correlates with the level of Ehrlichia antigens or antibodies in the sample.

Features

1. High Sensitivity and Specificity:
   • Detects low levels of Ehrlichia-specific antigens or antibodies, ensuring accurate diagnosis.
2. Rapid and Reliable:
   • Provides results in a short time frame, suitable for clinical and research settings.
3. Comprehensive Detection:
   • Suitable for identifying Ehrlichia infections at different stages of the disease.
4. Easy-to-Use:
   • Ready-to-use reagents and pre-coated plates simplify the workflow.
5. Quantitative and Qualitative Analysis:
   • Allows for precise quantification or qualitative assessment of infection status.

Kit Components

• Pre-coated Microelisa plate.
• Ehrlichia-specific standards or controls.
• HRP-conjugated detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Veterinary Diagnostics:
  • Diagnose Ehrlichiosis in dogs during routine check-ups or clinical disease presentations.
• Epidemiological Studies:
  • Monitor prevalence and distribution of Ehrlichia infections in canine populations.
• Treatment Monitoring:
  • Assess therapeutic efficacy and disease resolution in treated dogs.
• Research Applications:
  • Investigate host-pathogen interactions and immune responses to Ehrlichia canis.

Advantages

1. Accuracy:
   • Ensures minimal false positives or negatives due to high specificity.
2. Convenience:
   • All-in-one kit with pre-measured reagents reduces preparation time.
3. Versatility:
   • Suitable for various stages of Ehrlichiosis and a wide range of sample types.
4. Reproducibility:
   • Stringent quality control ensures consistent results.

Assay Protocol Summary

1. Add samples and controls to the pre-coated wells.
2. Incubate with HRP-conjugated detection antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Interpret results based on the provided standard curve or threshold values.

Storage and Stability

• Store the kit at 2–8°C for long-term stability.
• Avoid repeated freeze-thaw cycles of reagents to ensure integrity.

Limitations

• Species-Specific:
  • Designed specifically for canines and may not be suitable for other animals.
• For Research Use Only:
  • Not intended for human diagnostic purposes.

The Canine Ehrlichiosis ELISA Kit is a reliable and efficient tool for diagnosing Ehrlichia canis infections in dogs. Its sensitivity, specificity, and straightforward protocol make it a valuable resource for veterinary diagnostics, epidemiological research, and monitoring treatment responses.