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Sigma-Aldrich Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer, 5 x 1.5 mL
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Specifications:
| Application | PCR | ||
| Storage Temperature | 2-8°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Molecular Biology | Formula | CHCl₃ |
Sigma-Aldrich Chloroform is a high-purity organic solvent specifically suited for sensitive molecular biology applications, including PCR, RNA extraction, and DNA purification workflows. This chloroform reagent contains amylenes as stabilizers, providing stability during storage and consistent performance across nucleic acid extraction protocols.
It is colorless, has no detectable DNase or RNase activity, and is packaged in PCR-compatible vials to ensure convenience, contamination prevention, and ease of use in critical sample processing tasks.
Key Features & Benefits
- High Purity (≥99%): Ensures optimal performance in PCR and nucleic acid extraction workflows.
- PCR Suitability: Validated for use in molecular biology techniques including phenol-chloroform extraction.
- DNase/RNase-Free: Certified free of contaminating nucleases for safe use in RNA/DNA applications.
- Amylenes Stabilized: Prevents oxidative degradation, increasing shelf life and usability.
- Convenient Format: Supplied in 1.5 mL vials—ideal for bench use in molecular biology labs.
Applications
- Phenol-Chloroform Extraction: For purification of DNA and RNA from biological samples (e.g., blood culture fluids).
- RNA Extraction & Isolation: Used in classical organic extraction protocols.
- PCR Sample Preparation: Suitable for solvent-based purification steps to improve template quality.
- Organic Solvent for Laboratory Reactions: Can also serve in other analytical and preparative chemistry settings.
Chemical & Physical Properties
| Property | Value |
|---|---|
| Chemical Name | Chloroform (CHCl₃) |
| CAS Number | 67-66-3 |
| EC Number | 200-663-8 |
| MDL Number | MFCD00000826 |
| Molecular Weight | 119.38 g/mol |
| Boiling Point | 60.5–61.5 °C (lit.) |
| Melting Point | –63 °C (lit.) |
| Density (25 °C) | 1.492 g/mL |
| Vapor Pressure (20 °C) | 160 mmHg |
| Vapor Density (vs air) | 4.1 |
| Refractive Index (n20/D) | 1.445 (lit.) |
| Appearance | Colorless liquid |
| SMILES | ClC(Cl)Cl |
| InChI | 1S/CHCl3/c2-1(3)4/h1H |
| InChI Key | HEDRZPFGACZZDS-UHFFFAOYSA-N |
| Quality Level | 200 |
| Form | Liquid |
| Packaging | 1.5 mL vial |
Sigma-Aldrich Chloroform, ≥99%, PCR Reagent Grade is a high-quality organic solvent tailored for use in molecular biology applications where contamination-free and high-purity reagents are critical. Its superior formulation and stability make it a trusted choice for labs conducting nucleic acid extraction, purification, and sensitive downstream enzymatic applications like PCR and RT-PCR.