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Specifications:
| Application | Next Generation Sequencing, NGS Library Preparation |
| Storage Temperature | -20°C |
| Product Type | NGS library Prep kits |
| Product Brand | Hologic Diagenode |
| Product Grade | Molecular Biology |
The Hologic Diagenode 24 UDI for Tagmented Libraries – Set II (C01011036) is a unique dual-index (UDI) primer set designed to facilitate high-throughput multiplexed sequencing on Illumina platforms. This indexing solution enables simultaneous sequencing of up to 24 samples, reducing per-sample costs while ensuring high accuracy and minimal index hopping.
The Set II (C01011036) is part of a three-set system, which can be used independently for multiplexing 24 samples or combined with Set I (C01011034) and Set III (C01011037) to enable the multiplexing of up to 72 samples in a single sequencing run.
These UDIs are specifically optimized for tagmentation-based library preparation protocols, making them ideal for applications such as ChIPmentation, ATAC-seq, and CUT&Tag sequencing.
Key Features & Benefits
1. High-Throughput Multiplexing
- Enables multiplexing of up to 24 samples per sequencing run.
- Compatible with all three UDI sets, allowing multiplexing of up to 72 samples when combined.
2. Unique Dual-Indexing (UDI) for Enhanced Accuracy
- Reduces index hopping and misassignment of sequencing reads, improving data integrity.
- Ensures high specificity and sequencing accuracy, critical for ChIPmentation, ATAC-seq, and CUT&Tag applications.
3. Optimized for Tagmentation-Based Workflows
- Designed for seamless integration with tagmentation-based library preparation protocols, including:
- μChIPmentation for Histones (Cat. No. C01011011)
- ChIPmentation Kit for Histones (Cat. No. C01011009)
- TAG Kit for ChIPmentation (Cat. No. C01011030)
- ATAC-seq Kit (Cat. No. C01080002)
- Other tagmentation-based library preparation workflows, including CUT&Tag
Specifications
| Property | Details |
|---|---|
| Brand | Hologic Diagenode |
| Product Name | 24 UDI for Tagmented Libraries – Set II |
| Catalog Number | C01011036 |
| Multiplexing Capacity | 24 samples (Up to 72 when combined with Set I & III) |
| Index Type | Unique Dual Index (UDI) |
| Platform Compatibility | Illumina sequencing platforms |
| Application | ChIPmentation, ATAC-seq, CUT&Tag, tagmentation-based library prep |
Use Cases & Applications
1. Epigenomics & ChIP Sequencing
- Optimized for histone profiling and chromatin immunoprecipitation (ChIP) sequencing workflows.
- Allows multiplexing of multiple ChIP samples, increasing efficiency.
2. ATAC-seq & Chromatin Accessibility Studies
- Enables high-throughput sequencing of open chromatin regions.
- Ensures accurate indexing of multiple samples in ATAC-seq workflows.
3. CUT&Tag & Tagmentation-Based Library Preparation
- Compatible with CUT&Tag-based indexing and sequencing workflows.
- Reduces per-sample sequencing costs by allowing efficient sample pooling.
Ordering Information
| Catalog Number | Description | Multiplexing Capacity |
|---|---|---|
| C01011036 | 24 UDI for Tagmented Libraries – Set II | Up to 24 samples |
| C01011034 | 24 UDI for Tagmented Libraries – Set I | Up to 24 samples |
| C01011037 | 24 UDI for Tagmented Libraries – Set III | Up to 24 samples |
- All three UDI sets can be combined, allowing multiplexing of up to 72 samples in a single sequencing run.
The Hologic Diagenode 24 UDI for Tagmented Libraries – Set II (C01011036) is a high-precision, dual-index primer set designed for tagmentation-based library preparation workflows, ensuring accurate multiplexed sequencing with minimal index hopping. Compatible with Illumina platforms, this indexing solution is ideal for ChIPmentation, ATAC-seq, and CUT&Tag sequencing, making it a valuable tool for epigenomics and high-throughput sequencing applications.