Invitrogen™ NativePAGE™ Sample Prep Kit

The Invitrogen™ NativePAGE™ Sample Prep Kit is a complete reagent set designed for preparing protein samples for native gel electrophoresis. This kit contains optimized detergent solutions and buffers to improve the solubility of hydrophobic and membrane proteins, ensuring high-resolution separation in NativePAGE™ Novex Bis-Tris Gels.

With pre-measured, ready-to-use detergents (10% DDM and 5% Digitonin), this kit facilitates efficient membrane protein solubilization while preserving native protein structure, making it an essential tool for protein analysis in structural biology, proteomics, and functional studies.

Key Features & Benefits

1. Complete Sample Preparation Kit for NativePAGE™ Gels
   • Includes detergents, sample buffer, and G-250 sample additive for optimized native gel electrophoresis.
   • Reduces protein aggregation, improving resolution and minimizing streaking.
2. Optimized for Membrane & Hydrophobic Protein Solubilization
   • Contains 10% DDM (n-Dodecyl β-D-Maltoside) and 5% Digitonin for gentle yet effective protein extraction.
   • Preserves native protein conformation for functional and structural analysis.
3. Enhances Electrophoresis Performance & Protein Band Clarity
   • Minimizes sample precipitation and streaking in native gels.
   • Compatible with NativePAGE™ Novex Bis-Tris Gel System for high-resolution protein separation.
4. Pre-Measured, Ready-to-Use Reagents for Convenience
   • Eliminates detergent preparation errors, ensuring consistent protein solubilization.
   • Saves time and effort in preparing complex membrane protein samples.
5. Compatible with a Wide Range of Protein Research Applications
   • Suitable for cryo-EM, X-ray crystallography, mass spectrometry, and functional assays.
   • Ensures protein-protein interactions remain intact for biochemical analysis.

Product Specifications

Kit Components & Their Functions

1. 10% DDM (n-Dodecyl β-D-Maltoside) – 1 mL
   • A non-ionic detergent that solubilizes membrane proteins while preserving functionality.
   • Ideal for native protein extraction and structural studies.
2. 5% Digitonin – 1 mL
   • A mild detergent that maintains protein-lipid interactions.
   • Facilitates membrane protein solubilization for native gel analysis.
3. NativePAGE™ 4X Sample Buffer – 10 mL
   • Ensures proper protein loading and electrophoretic mobility.
   • Maintains protein integrity during electrophoresis.
4. NativePAGE™ 5% G-250 Sample Additive – 0.5 mL
   • Used for anodic gel staining and sample preparation.
   • Improves protein visualization and band separation.

Use Cases & Applications

1. Native Gel Electrophoresis & Protein Analysis

• Enhances Protein Resolution in Native Electrophoresis
  • Prevents protein aggregation and streaking in gels.
  • Ensures sharp, well-defined protein bands for downstream analysis.
• Ideal for Studying Protein Complexes & Structural Biology
  • Preserves protein-protein interactions in native conditions.
  • Enables functional and conformational analysis of protein complexes.

2. Membrane Protein Extraction & Functional Studies

• Maintains Membrane Protein Conformation & Activity
  • Provides gentle solubilization without denaturing proteins.
  • Suitable for G-protein-coupled receptors (GPCRs), ion channels, and transporters.
• Compatible with Cryo-EM & Structural Biology Research
  • Used for detergent exchange in cryo-EM sample preparation.
  • Helps preserve native lipid environment in protein complexes.

3. Protein Purification & Biochemical Assays

• Facilitates Native Protein Sample Preparation for Mass Spectrometry
  • Ensures solubilized proteins remain intact during MS analysis.
  • Reduces sample loss and degradation.
• Used in Enzyme Kinetics & Functional Activity Studies
  • Maintains enzyme activity by preserving natural folding.
  • Helps study protein-protein interactions in physiological conditions.

4. Lipid Raft & Cholesterol Research

• Preserves Lipid-Protein Interactions in Cellular Studies
  • Extracts cholesterol-rich domains while preserving protein activity.
  • Used in studies of lipid rafts and signaling pathways.

Instructions for Use

Protein Extraction & Sample Preparation:

1. Solubilization of Membrane Proteins:
   • Use 10% DDM or 5% Digitonin at recommended concentrations (1–2% w/v).
   • Incubate with protein lysates for 30–60 minutes at 4°C.
   • Centrifuge at 100,000 x g for 1 hour and collect the supernatant.
2. Native Electrophoresis Sample Prep:
   • Mix solubilized proteins with NativePAGE™ 4X Sample Buffer.
   • Add NativePAGE™ 5% G-250 Sample Additive before loading into the gel.
   • Load prepared samples onto NativePAGE™ Novex Bis-Tris Gels and run electrophoresis.

Storage & Handling Instructions

• Storage Conditions:
  • Store at 2–8°C for long-term stability.
  • Do not freeze to maintain reagent integrity.
• Handling Precautions:
  • Use aseptic techniques to prevent contamination.
  • Ensure all reagents are mixed properly before use.
• Disposal Guidelines:
  • Dispose of detergent-containing solutions as per institutional chemical waste regulations.

Why Choose Invitrogen™ NativePAGE™ Sample Prep Kit?

✅ Complete detergent set for native protein solubilization & electrophoresis

✅ Compatible with NativePAGE™ Novex Bis-Tris Gel System

✅ Reduces streaking & improves protein resolution in native gels

✅ Maintains protein integrity for functional & structural analysis

✅ Pre-measured, ready-to-use reagents for consistent results

Invitrogen™ NativePAGE™ Sample Prep Kit is a powerful tool for membrane protein extraction, native electrophoresis, and biochemical research, ensuring high-quality results for protein function and structural studies.