Invitrogen™ DDM (n-dodecyl β-D-maltoside) (10%), 1mL

Invitrogen™ DDM (n-Dodecyl β-D-Maltoside) (10%) is a high-purity, non-ionic detergent solution designed for the solubilization and stabilization of membrane proteins in native conditions. DDM is widely used in native protein sample preparation, particularly for membrane protein extraction, structural biology studies, and electrophoresis applications.

This ready-to-use 10% DDM solution is optimized for NativePAGE™ Novex Bis-Tris Gels and the NativePAGE™ Sample Prep Kit, ensuring improved solubility, enhanced protein resolution, and reduced streaking in native gel electrophoresis.

Key Features & Benefits

1. Non-Ionic Detergent for Native Protein Stabilization
   • Maintains protein activity and structural integrity.
   • Reduces protein aggregation, improving solubilization.
2. Optimized for Membrane Protein Extraction & Solubilization
   • Ideal for hydrophobic and transmembrane protein purification.
   • Works at low concentrations to preserve native protein conformation.
3. Compatible with Native Gel Electrophoresis & Structural Studies
   • Used in NativePAGE™ Gel System for improved band resolution.
   • Included in NativePAGE™ Sample Prep Kit for membrane protein solubilization.
4. Ready-to-Use 10% DDM Solution
   • Pre-dissolved detergent solution eliminates preparation time.
   • Stored at 4°C, ensuring long-term stability.
5. Compatible with Various Protein Research Applications
   • Used in cryo-EM, X-ray crystallography, mass spectrometry, and enzyme assays.
   • Helps maintain lipid-protein interactions in membrane biology studies.

Product Specifications

Use Cases & Applications

1. Membrane Protein Solubilization & Structural Biology

• Preserves Native Membrane Protein Conformation
  • Extracts membrane proteins while maintaining their biological activity.
  • Commonly used for ion channels, transporters, and G-protein-coupled receptors (GPCRs).
• Essential for Cryo-EM & X-ray Crystallography Studies
  • Helps stabilize protein-lipid complexes for high-resolution structural analysis.
  • Used in detergent exchange strategies for cryo-EM grid preparation.

2. NativePAGE™ Gel Electrophoresis & Protein Analysis

• Enhances Resolution & Solubility in Native Electrophoresis
  • Included in NativePAGE™ Sample Prep Kit for high-resolution separation.
  • Works in combination with 5% Digitonin for optimized banding patterns.
• Prevents Protein Aggregation & Streaking in Gels
  • Ensures even migration of membrane proteins in native gels.
  • Improves quantitative protein analysis and downstream characterization.

3. Detergent-Based Protein Purification & Biochemical Assays

• Used in Chromatography for Membrane Protein Isolation
  • Compatible with affinity purification (His-tag, FLAG-tag), SEC, and ion exchange chromatography.
  • Helps retain protein functionality during elution and purification.
• Stabilizes Enzymes & Protein Complexes in Functional Studies
  • Reduces denaturation and degradation of sensitive proteins.
  • Maintains protein-protein and protein-lipid interactions for enzyme kinetics.

4. Lipid Raft & Cholesterol Research

• Preserves Lipid-Protein Interactions in Cellular Studies
  • Extracts cholesterol-rich domains while preserving protein activity.
  • Used in studies of lipid rafts and signaling pathways.
• Detergent-Based Sample Preparation for Mass Spectrometry
  • Compatible with MS analysis of membrane proteins.
  • Ensures minimal interference with ionization in mass spectrometry workflows.

Instructions for Use

Protein Extraction & Sample Preparation:

1. Solubilization of Membrane Proteins:
   • Dilute 10% DDM solution to 1–2% (w/v) in the desired buffer.
   • Incubate with membrane protein samples at 4°C for 30–60 min.
   • Centrifuge at 100,000 x g for 1 hour and collect the supernatant.
2. Native Electrophoresis Sample Prep:
   • Mix DDM with protein lysates for solubilization.
   • Load prepared samples onto NativePAGE™ Novex Bis-Tris Gels.
3. Cryo-EM & Structural Biology:
   • Use DDM in protein purification and detergent exchange protocols.
   • Prepare grid samples with controlled detergent concentration to optimize particle distribution.

Storage & Handling Instructions

• Storage Conditions:
  • Store at 4°C for long-term stability.
  • Avoid freezing, as repeated freeze-thaw cycles may alter detergent properties.
• Handling Precautions:
  • Use aseptic techniques to prevent contamination.
  • Prepare fresh working solutions for optimal performance.
• Disposal Guidelines:
  • Dispose of DDM-containing solutions according to institutional chemical waste protocols.

Why Choose Invitrogen™ DDM (10%) Solution?

✅ Non-ionic detergent for mild solubilization of membrane proteins

✅ Optimized for cryo-EM, X-ray crystallography, and mass spectrometry workflows

✅ Enhances resolution and solubility in NativePAGE™ gel electrophoresis

✅ Prevents aggregation and preserves protein functionality

✅ Pre-dissolved 10% solution for convenient, ready-to-use applications

Invitrogen™ DDM (10%) Solution is a versatile detergent for protein research, providing superior membrane protein solubilization, sample preparation for electrophoresis, and structural biology applications.