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Thermo Scientific™ Invitrogen™ Human IL-12 p70 ELISA Kit
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Assay Range 3.1-200 pg/mL
Assay Sensitivity 2.1 pg/mL
Specifications:
| Application | ELISA-Based Assays |
| Storage Temperature | 2-8°C |
| Product Type | Elisa Kit |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Thermo Scientific™ Invitrogen™ Human IL-12 p70 ELISA Kit is a robust, high-sensitivity assay designed for the quantitative detection of Interleukin-12 p70 (IL-12 p70) in human biological samples, including serum, plasma, buffered solutions, and cell culture media. This sandwich ELISA kit provides an efficient, accurate, and specific method for measuring IL-12 p70 levels, a critical cytokine in immune response regulation.
Key Features
- High Sensitivity and Precision:
- Analytical Sensitivity: Detects as low as 2.1 pg/mL.
- Assay Range: 3.1–200 pg/mL.
- Intraassay CV: 3%.
- Interassay CV: 4.8%.
- Versatile Sample Compatibility:
- Suitable for human serum, plasma, and cell culture supernatants.
- Requires only 50 µL of sample.
- Comprehensive Kit Design:
- Includes pre-coated 96-well plates, standards, controls, and all necessary reagents.
- Reliable Sandwich ELISA Format:
- Ensures specific detection of natural and recombinant human IL-12 p70 using a pre-coated capture antibody and a biotinylated detection antibody.
- Time-Efficient Workflow:
- Hands-on time: 1 hr 20 min.
- Total time-to-result: 3 hr 10 min.
Applications and Use Cases
1. Immunology Research:
- Immune Response Studies:
- Quantify IL-12 p70 to explore its role in T cell and NK cell activation.
- Cytokine Profiling:
- Assess IL-12 p70 levels as part of broader cytokine profiling in immune disorders.
2. Clinical Research:
- Infectious Disease Monitoring:
- Measure IL-12 p70 levels in response to bacterial, viral, or parasitic infections.
- Autoimmune Disease Studies:
- Investigate IL-12 p70's role in modulating inflammatory responses in autoimmune conditions like rheumatoid arthritis and lupus.
3. Cancer Research:
- Therapeutic Development:
- Evaluate IL-12 p70 as a potential therapeutic target for enhancing anti-tumor immunity.
- Tumor Microenvironment Analysis:
- Quantify IL-12 p70 to study its role in immune modulation within the tumor microenvironment.
4. Vaccine Development:
- Adjuvant Studies:
- Assess IL-12 p70 production as a marker for immune activation following vaccine administration.
Kit Components
| Component | Description |
|---|---|
| Pre-Coated 96-Well Plate | Immobilized with a specific antibody to IL-12 p70. |
| Standards | For generating a standard curve for quantification. |
| Sample Diluent | Optimizes sample preparation. |
| Assay Buffer Concentrate | Enhances assay performance. |
| Biotinylated Detection Antibody | Specifically binds to IL-12 p70. |
| SAV-HRP | Amplifies the detection signal. |
| Wash Buffer | Removes unbound substances. |
| Controls | Ensures assay accuracy and reproducibility. |
| TMB Chromogen | Produces a colorimetric signal. |
| Stop Solution | Stabilizes the colorimetric signal for OD measurement. |
| Adhesive Plate Covers | Prevents evaporation during incubation. |
Assay Principle
The Human IL-12 p70 ELISA Kit uses a sandwich ELISA method for accurate quantification:
- Capture Phase:
- A pre-coated antibody in the 96-well plate captures IL-12 p70 from the sample.
- Detection Phase:
- A biotinylated detection antibody binds to a different epitope of the IL-12 p70 molecule, forming a sandwich complex.
- Signal Development:
- SAV-HRP binds to the biotinylated detection antibody, and TMB substrate is added to produce a colorimetric signal.
- Quantification:
- Measure the optical density (OD) at 450 nm to calculate IL-12 p70 concentrations using the standard curve.
Performance Specifications
| Feature | Details |
|---|---|
| Detection Method | Colorimetric |
| Dynamic Range | 3.1–200 pg/mL |
| Sensitivity | 2.1 pg/mL |
| Sample Volume Required | 50 µL |
| Species Reactivity | Human |
| Pack Size | 96 Tests |
Storage and Shipping
- Storage Conditions: Store kit components at 2–8°C.
- Shipping Conditions: Shipped on wet ice to maintain reagent integrity.
Target Information: Interleukin-12 p70 (IL-12 p70)
- Function:
- A pro-inflammatory cytokine that plays a key role in activating T cells and NK cells.
- Stimulates the production of interferon-gamma (IFN-γ) to enhance immune responses.
- Source:
- Produced by dendritic cells, macrophages, and B cells in response to infections or tumors.
- Clinical Significance:
- Dysregulated IL-12 p70 levels are associated with autoimmune diseases, infectious diseases, and cancer.
- Gene Information:
- IL12A (P35) and IL12B (P40) subunits encode the active IL-12 p70 heterodimer.
Advantages of the Kit
- High Sensitivity and Specificity:
- Detects low levels of IL-12 p70 with minimal cross-reactivity.
- Validated Performance:
- Rigorous lot-to-lot testing ensures consistent results.
- Ease of Use:
- Ready-to-use reagents simplify assay setup and minimize handling errors.
- Broad Applications:
- Suitable for immunology, cancer research, and vaccine studies.
The Thermo Scientific™ Invitrogen™ Human IL-12 p70 ELISA Kit provides a robust, sensitive, and accurate platform for quantifying IL-12 p70 in various human biological samples. Its high precision, ease of use, and broad applicability make it an essential tool for researchers investigating immune responses, disease mechanisms, and therapeutic interventions.