0
Invitrogen™ BacLight™ Bacterial Membrane Potential Kit
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | Flow Cytometry, Cell Analysis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Flow Cytometry Assay Kit for Real-Time Detection of Bacterial Membrane Potential
The Invitrogen™ BacLight™ Bacterial Membrane Potential Kit provides a simple and sensitive method for evaluating bacterial membrane potential using flow cytometry. This kit includes the fluorescent dye DiOC2(3), which accumulates in bacterial cells in a potential-dependent manner, enabling clear differentiation between energized and depolarized cells. The proton ionophore CCCP is included as a control reagent to collapse membrane potential, providing a reliable negative control.
The dual-color readout (green for all bacteria, red for polarized cells) enables precise analysis of membrane integrity, making this kit ideal for bacterial viability studies, antibiotic mode-of-action testing, and physiological assessments.
Key Features and Benefits
Dual Fluorescence Readout for Membrane Potential
- DiOC2(3) stains all bacterial cells green, but in polarized (viable) cells, the dye self-associates and shifts fluorescence emission to red.
- Easily distinguishes between live (energized) and depolarized bacterial populations.
Ready-to-Use Control Reagent Included
- CCCP (carbonyl cyanide m-chlorophenyl hydrazone) serves as a potent proton gradient disruptor to validate assay performance and gating strategy.
Flow Cytometry Compatible
- Designed for flow cytometers equipped with standard FITC channels (excitation 488 nm; emission 497 nm green, red shift observed upon aggregation).
- Enables rapid, real-time membrane potential measurements with minimal sample preparation.
Reliable and Convenient Format
- Includes pre-formulated buffer and concentrated reagents for up to 100 tests.
- All components are supplied in light-protective packaging to preserve fluorescence quality.
Applications
- Assessment of bacterial viability based on membrane polarization
- Antibiotic efficacy testing via disruption of membrane potential
- Drug mechanism of action and resistance profiling
- Flow cytometry-based live/dead differentiation in bacterial populations
- Bacterial physiology research and metabolic studies
Technical Specifications
| Property | Details |
|---|---|
| Catalog Number | B34950 |
| Detection Method | Fluorescence |
| Dye Type | Membrane Potential Probe – DiOC2(3) |
| Excitation / Emission | 482 nm / 497 nm (green); red shift in polarized cells |
| Format | Tubes |
| Compatible Equipment | Flow cytometer |
| Assay Type | Bacterial Membrane Potential Assay |
| Shipping Condition | Wet ice |
| Storage Condition | 2–8°C; protect from light |
| Product Line | BacLight™ |
| Unit Size | 1 kit |
| Contents | DiOC2(3), 3 mM in DMSO (1.2 mL); CCCP, 500 μM in DMSO (300 µL); PBS (200 mL) |
The Invitrogen™ BacLight™ Bacterial Membrane Potential Kit offers a fast, sensitive, and flow cytometry-compatible approach for evaluating bacterial cell health through membrane potential analysis. Its dual-color fluorescence strategy, combined with the included control reagent, makes it a powerful tool for microbial research, drug development, and antibiotic testing.