Autobio Mycoplasma IES Detection Kits

​The Autobio Mycoplasma IES Detection Kits are rapid molecular diagnostic tools for the qualitative detection of Mycoplasma infections, including Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH).

These pathogens are among the main causes of nongonococcal urethritis (NGU), cervicitis, pelvic inflammatory disease (PID), orchitis, epididymitis, and infertility in both men and women.

Due to the increasing prevalence and antibiotic resistance of Mycoplasma species, timely detection is essential for accurate diagnosis and effective treatment.

The Autobio IES platform (Isothermal Enzymatic System) allows rapid amplification and detection of Mycoplasma DNA without the need for complex thermal cycling, offering a simple, fast, and highly sensitive diagnostic solution.

Available Kit Variants

Key Features

• Rapid detection: Results available in less than 60 minutes.
• Broad coverage: Detects multiple Mycoplasma species including U. urealyticum and M. hominis.
• High specificity and sensitivity: Minimizes false positives from interfering organisms.
• Simple workflow: Easy sample processing and result interpretation with ready-to-use reagents.
• Comprehensive detection system: Incorporates inhibition agents to suppress non-Mycoplasma microbial growth.
• No need for complex instruments: Operates at a constant temperature via isothermal amplification.
• Ideal for clinical and laboratory testing: Detects pathogens associated with reproductive tract infections and cell culture contamination.

Clinical Significance

Mycoplasma species, particularly Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH), are recognized as key pathogens in genitourinary infections.

These microorganisms can attack and destroy epithelial cells, facilitating co-infection with HIV and other sexually transmitted diseases, and may cause infertility in both sexes due to their impact on sperm motility and reproductive tract inflammation.

With the rise of antimicrobial resistance (AMR) due to widespread misuse of antibiotics, rapid and accurate detection of Mycoplasma is crucial for clinical management and treatment optimization.

Kit Components

1. Reaction Strip – For sample loading and detection.
2. Lyophilized Powder – Contains growth inhibition agents to suppress non-Mycoplasma organisms while promoting Mycoplasma growth.
3. Mineral Oil – Prevents evaporation and stabilizes reaction conditions.
4. Diluent – Used to dissolve the lyophilized powder and reconstitute the reaction mix.

Technical Specifications

Applications

• Clinical Microbiology: Rapid detection of Mycoplasma infections in urogenital samples.
• Reproductive Health Clinics: Screening for Mycoplasma-related infertility and pelvic infections.
• Cell Culture Laboratories: Routine monitoring of Mycoplasma contamination in cell lines and bioproducts.
• Public Health and STD Clinics: Fast and reliable pathogen detection for epidemiological screening.
• Pharmaceutical Quality Control: Ensures Mycoplasma-free raw materials and biologics.

Unique Advantages

• Fast, accurate, and simple: Provides rapid and reproducible results.
• Comprehensive pathogen coverage: Detects major reproductive pathogens (UU and MH).
• Enhanced specificity: Inhibition agent eliminates false positives caused by other bacteria.
• Ideal for low-resource settings: Requires minimal instrumentation and user training.
• Suitable for both clinical and research applications.

References

1. Núñez-Calonge R, Caballero P, Redondo C, et al. Ureaplasma urealyticum reduces motility and induces membrane alterations in human spermatozoa. Hum Reprod. 1998;13(10):2756–2761.
2. Rylander M, Hallander HO. *In vitro comparison of doxycycline, tetracycline, erythromycin, and a new macrolide against Mycoplasma pneumoniae, M. hominis, and U. urealyticum. Scand J Infect Dis Suppl. 1988;53:12–17.
3. Murray P. Manual of Clinical Microbiology. 9th ed. Washington, D.C.: ASM Press; 2007.

The Autobio Mycoplasma IES Detection Kits provide laboratories and clinics with a rapid, specific, and easy-to-use solution for detecting Mycoplasma infections, including Ureaplasma urealyticum and Mycoplasma hominis.

By leveraging isothermal amplification technology and advanced inhibition-based selectivity, these kits ensure accurate detection while reducing false positives, enabling clinicians to make timely diagnostic and therapeutic decisions.

A trusted choice for clinical, reproductive health, and cell culture laboratories seeking reliable Mycoplasma screening solutions.