Applied Biosystems™ TaqMan™ Gene Expression Assay (FAM)

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.

Features of TaqMan Gene Expression assays include:

• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required

• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay

• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets

• Accurate—identify small fold-changes with high accuracy of quantitation

• Extensive content—Over 1.8 million predesigned assays available for over 25 different species

• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology

• Fast delivery—your assays shipped in as little as 1–2 days (see below)

Approximate ship time

• Inventoried: 1–2 days in North America, 3–5 days in Europe*

• Made to order: 4–6 days in North America, 6–10 days in Europe

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Pre-formulated assay

• 2 unlabeled PCR primers (900 nM each final 1X concentration)

• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

Recommended master mix: TaqMan Fast Advanced Master Mix