Apollo Sci. Pepsin Enzyme

Apollo Scientific Pepsin Enzyme is a proteolytic enzyme commonly used in biochemical and pharmaceutical research to digest proteins into peptides. Derived from the stomach lining of animals, particularly pigs, pepsin is active under acidic conditions and is often used in protein analysis, cell culture preparation, and digestive studies.

Apollo Scientific Pepsin Enzyme

1. Product Overview
   • Enzyme Name: Pepsin
   • Source: Typically derived from porcine gastric mucosa.
   • CAS Number: 9001-75-6
   • Activity: The enzyme’s activity is measured in units (often expressed as U/mg), indicating its capacity to break down proteins at a specific rate.
2. Physical Properties
   • Appearance: White to off-white, fine powder or crystalline form.
   • Solubility: Soluble in acidic solutions (e.g., HCl) and usually insoluble in water at neutral pH.
   • Storage Conditions: Store in a cool, dry place, often recommended at -20°C to preserve enzyme activity over time.
3. Optimal Conditions
   • Optimal pH: Active in acidic environments, with peak activity around pH 1.5 to 2.5.
   • Temperature: Optimal temperature for activity is typically 37°C (body temperature), though it can vary slightly depending on the specific preparation.
4. Applications
   • Protein Digestion and Hydrolysis: Used in protein studies to digest proteins into smaller peptides for analysis.
   • Biochemical Research: Assists in preparing protein samples, enzyme kinetics studies, and digestion assays.
   • Pharmaceuticals and Supplements: Used as a digestive aid in formulations to help break down protein-rich food in the stomach.
   • Cell and Tissue Culture Preparation: Often utilized in the dissociation of tissues in cell culture protocols.
5. Safety and Handling
   • Hazards: May cause irritation if inhaled or if it comes into contact with skin or eyes; handle with gloves and a mask.
   • Storage: Should be stored in tightly sealed containers to avoid moisture and contamination.
6. Packaging
   • Available in various quantities, often tailored to laboratory research or industrial applications.

Benefits

• Highly Specific Protease: Breaks down only certain peptide bonds, making it useful in controlled protein hydrolysis.
• Versatile: Applicable across biochemistry, pharmaceuticals, and food industries for protein digestion.
• Stable and Long-Lasting: When stored properly, pepsin retains activity for extended periods, providing consistent results.

Pepsin from Apollo Scientific is valued for its reliability in controlled enzymatic digestion, making it ideal for studies that require precise protein breakdown