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![Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] - BSA and Azide free, 100µL](/web/image/product.product/47803/image_1024/%5Bab281303%5D%20Abcam%20Anti-SARS-CoV-2%20Spike%20RBD%20antibody%20%5BHL1003%5D%20-%20BSA%20and%20Azide%20free%2C%20100%C2%B5L?unique=8ecb4d2)
![Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] - BSA and Azide free, 100µL](/web/image/product.image/2473/image_1024/Abcam%20Anti-SARS-CoV-2%20Spike%20RBD%20antibody%20%5BHL1003%5D%20-%20BSA%20and%20Azide%20free%2C%20100%C2%B5L?unique=d565781)
![Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] - BSA and Azide free, 100µL](/web/image/product.image/2474/image_1024/Abcam%20Anti-SARS-CoV-2%20Spike%20RBD%20antibody%20%5BHL1003%5D%20-%20BSA%20and%20Azide%20free%2C%20100%C2%B5L?unique=d565781)
![Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] - BSA and Azide free, 100µL](/web/image/product.image/2475/image_1024/Abcam%20Anti-SARS-CoV-2%20Spike%20RBD%20antibody%20%5BHL1003%5D%20-%20BSA%20and%20Azide%20free%2C%20100%C2%B5L?unique=d565781)
![Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] - BSA and Azide free, 100µL](/web/image/product.product/47803/image_128/%5Bab281303%5D%20Abcam%20Anti-SARS-CoV-2%20Spike%20RBD%20antibody%20%5BHL1003%5D%20-%20BSA%20and%20Azide%20free%2C%20100%C2%B5L?unique=8ecb4d2)
![Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] - BSA and Azide free, 100µL](/web/image/product.image/2473/image_128/Abcam%20Anti-SARS-CoV-2%20Spike%20RBD%20antibody%20%5BHL1003%5D%20-%20BSA%20and%20Azide%20free%2C%20100%C2%B5L?unique=d565781)
![Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] - BSA and Azide free, 100µL](/web/image/product.image/2474/image_128/Abcam%20Anti-SARS-CoV-2%20Spike%20RBD%20antibody%20%5BHL1003%5D%20-%20BSA%20and%20Azide%20free%2C%20100%C2%B5L?unique=d565781)
![Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] - BSA and Azide free, 100µL](/web/image/product.image/2475/image_128/Abcam%20Anti-SARS-CoV-2%20Spike%20RBD%20antibody%20%5BHL1003%5D%20-%20BSA%20and%20Azide%20free%2C%20100%C2%B5L?unique=d565781)
Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] - BSA and Azide free, 100µL
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | Protein Assays & Analysis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Recombinant Protein | Forms | Liquid |
| Product Brand | abcam | ||
| Product Grade | Molecular Biology | ||
The Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] (ab281303) is a rabbit recombinant monoclonal IgG antibody specifically targeting the receptor-binding domain (RBD) of the SARS-CoV-2 Spike protein. This carrier-free (BSA- and azide-free) antibody is optimized for functional, immunocytochemical, and immunoassay applications, making it highly suitable for neutralization studies, assay development, and advanced COVID-19 research.
Produced using recombinant technology, this antibody delivers exceptional batch-to-batch consistency, high specificity, and reproducible performance. It has been functionally validated to inhibit infection of mammalian cells by live SARS-CoV-2, demonstrating its suitability for neutralization and blocking studies.
Key Features
- Recombinant rabbit monoclonal antibody for superior consistency
- Specific for SARS-CoV-2 Spike protein RBD
- BSA- and azide-free (carrier-free) – ideal for functional and conjugation applications
- Validated for neutralization and blocking of live SARS-CoV-2 infection
- High purity via Protein A affinity purification
- Suitable for research, assay development, and functional studies
Applications
Validated for use in:
- Functional Studies (Neutralization / Blocking assays)
- Immunocytochemistry (ICC)
- Sandwich ELISA (sELISA)
- Indirect ELISA (I-ELISA)
This antibody is cited in peer-reviewed literature and has been experimentally validated across multiple assay platforms.
Reactivity
- Species Reactivity: SARS-CoV-2
- Specificity: Spike protein – Receptor Binding Domain (RBD)
Recommended Application Dilutions
| Application | Species | Dilution / Notes |
|---|---|---|
| ICC | SARS-CoV-2 | 1:100 – 1:1000 |
| sELISA | SARS-CoV-2 | Tested |
| FuncS (Neut/Block) | SARS-CoV-2 | Tested – inhibits live virus infection |
| I-ELISA | SARS-CoV-2 | Tested |
Antibody Characteristics
| Parameter | Description |
|---|---|
| Product Name | Anti-SARS-CoV-2 Spike RBD antibody |
| Clone | HL1003 |
| Catalog Number | ab281303 |
| Host Species | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Recombinant | Yes |
| Carrier Free | Yes |
| Concentration | ~1 mg/mL (batch dependent) |
| Form | Liquid |
| Purification | Protein A affinity purification |
Storage & Handling
- Storage Buffer: PBS, pH 7.4
- Shipping Condition: Blue ice
- Short-term Storage: +4 °C
- Long-term Storage: +4 °C
- Avoid repeated freeze–thaw cycles
Applications & Research Use
- SARS-CoV-2 neutralization assays
- COVID-19 vaccine and therapeutic research
- Viral entry and Spike-ACE2 interaction studies
- Diagnostic assay development
- Immunological and virology research
Research Use Only (RUO). Not for diagnostic or therapeutic use.
The Abcam Anti-SARS-CoV-2 Spike RBD antibody [HL1003] (ab281303) is a highly specific, functionally validated recombinant monoclonal antibody ideal for neutralization, immunocytochemistry, and ELISA-based SARS-CoV-2 research. Its carrier-free formulation and proven blocking capability make it an excellent choice for advanced virology, immunology, and assay development workflows.