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Next-generation transfection reagent optimized for Sf9 and Sf21 insect cells
The Gibco™ ExpiFectamine™ Sf Transfection Reagent is a high-performance, cationic lipid-based reagent engineered for efficient transfection of insect cells, particularly Sf9 and Sf21 cell lines. Optimized as a core component of the ExpiSf™ Expression System, it enables rapid, reproducible delivery of plasmid DNA into insect cells for the production of high-titer recombinant baculovirus (P0) without the need for virus amplification.
This reagent supports both adherent and suspension cultures, with a simplified workflow that includes a short 5-minute complexation time, low cytotoxicity, and no post-transfection medium change, ensuring maximum efficiency and user convenience.
Key Features
- Robust & Reproducible: Delivers consistent transfection performance for confident experimental results.
- P0 Baculovirus Production: Enables efficient generation of high-quality, high-titer P0 recombinant baculovirus.
- Flexible Culture Compatibility: Effective in adherent and suspension cultures of Sf9 and Sf21 cells.
- Low Toxicity: Gentle on cells; medium replacement after transfection is not required.
- Fast Workflow: Features a 5-minute DNA complexation step for rapid protocol execution.
- Scalable: Linearly scalable transfection from 4 mL to 500 mL culture volumes in suspension format.
- Compatible Media: Validated with ExpiSf CD Medium, Sf-900™ II SFM, and Sf-900™ III SFM.
Applications
- Transient transfection of insect cells (Sf9, Sf21)
- Recombinant baculovirus (P0) generation
- Gene expression studies in insect cell systems
- Protein production using the baculovirus expression vector system (BEVS)
Technical Specifications
| Specification | Details |
|---|---|
| Product Type | Transfection Reagent |
| Transfection Technique | Lipid-based |
| Cell Type | Insect cells (Sf9, Sf21) |
| Sample Type | Plasmid DNA, Baculovirus DNA |
| Serum Compatible | Yes |
| High-throughput Compatible | Yes |
| Product Line | ExpiFectamine™ |
| Shipping Conditions | Room temperature or wet ice |
| Storage Conditions | Store at 2–8°C, do not freeze |
| Shelf Life | 24 months from date of manufacture |
| Unit Size | 1 mL |
| Catalog Number | A38915 |
The Gibco™ ExpiFectamine™ Sf Transfection Reagent is an essential tool for researchers using baculovirus-based protein expression in insect cell systems. Its ease of use, high efficiency, and compatibility with both suspension and adherent cultures make it a superior choice for labs requiring reproducible, scalable transfection solutions.
User Guide: ExpiFectamine Sf Transfection Reagent
Next-generation transfection reagent optimized for Sf9 and Sf21 insect cells
The Gibco™ ExpiFectamine™ Sf Transfection Reagent is a high-performance, cationic lipid-based reagent engineered for efficient transfection of insect cells, particularly Sf9 and Sf21 cell lines. Optimized as a core component of the ExpiSf™ Expression System, it enables rapid, reproducible delivery of plasmid DNA into insect cells for the production of high-titer recombinant baculovirus (P0) without the need for virus amplification.
This reagent supports both adherent and suspension cultures, with a simplified workflow that includes a short 5-minute complexation time, low cytotoxicity, and no post-transfection medium change, ensuring maximum efficiency and user convenience.
Key Features
- Robust & Reproducible: Delivers consistent transfection performance for confident experimental results.
- P0 Baculovirus Production: Enables efficient generation of high-quality, high-titer P0 recombinant baculovirus.
- Flexible Culture Compatibility: Effective in adherent and suspension cultures of Sf9 and Sf21 cells.
- Low Toxicity: Gentle on cells; medium replacement after transfection is not required.
- Fast Workflow: Features a 5-minute DNA complexation step for rapid protocol execution.
- Scalable: Linearly scalable transfection from 4 mL to 500 mL culture volumes in suspension format.
- Compatible Media: Validated with ExpiSf CD Medium, Sf-900™ II SFM, and Sf-900™ III SFM.
Applications
- Transient transfection of insect cells (Sf9, Sf21)
- Recombinant baculovirus (P0) generation
- Gene expression studies in insect cell systems
- Protein production using the baculovirus expression vector system (BEVS)
Technical Specifications
| Specification | Details |
|---|---|
| Product Type | Transfection Reagent |
| Transfection Technique | Lipid-based |
| Cell Type | Insect cells (Sf9, Sf21) |
| Sample Type | Plasmid DNA, Baculovirus DNA |
| Serum Compatible | Yes |
| High-throughput Compatible | Yes |
| Product Line | ExpiFectamine™ |
| Shipping Conditions | Room temperature or wet ice |
| Storage Conditions | Store at 2–8°C, do not freeze |
| Shelf Life | 24 months from date of manufacture |
| Unit Size | 1 mL |
| Catalog Number | A38915 |
The Gibco™ ExpiFectamine™ Sf Transfection Reagent is an essential tool for researchers using baculovirus-based protein expression in insect cell systems. Its ease of use, high efficiency, and compatibility with both suspension and adherent cultures make it a superior choice for labs requiring reproducible, scalable transfection solutions.
![[A35243] Gibco™ ExpiSf9™ Cells, 1.0 x 107 cells/mL](/web/image/product.product/43799/image_512/%5BA35243%5D%20Gibco%E2%84%A2%20ExpiSf9%E2%84%A2%20Cells%2C%201.0%20x%20107%20cells-mL?unique=96c5da2)
![[A3767801] Gibco™ ExpiSf™ CD Medium (500 mL)](/web/image/product.product/43801/image_512/%5BA3767801%5D%20Gibco%E2%84%A2%20ExpiSf%E2%84%A2%20CD%20Medium%20%28500%20mL%29?unique=96c5da2)
![[A3767806] Gibco™ ExpiSf™ Protein Production Kit (1 L (ExpiSf CD Medium), 4 x 1 mL (ExpiSf Enhancer))](/web/image/product.product/43806/image_512/%5BA3767806%5D%20Gibco%E2%84%A2%20ExpiSf%E2%84%A2%20Protein%20Production%20Kit%20%281%20L%20%28ExpiSf%20CD%20Medium%29%2C%204%20x%201%20mL%20%28ExpiSf%20Enhancer%29%29?unique=96c5da2)
![[A38841] Gibco™ ExpiSf™ Expression System Starter Kit with Erlenmeyer Flasks](/web/image/product.product/43810/image_512/%5BA38841%5D%20Gibco%E2%84%A2%20ExpiSf%E2%84%A2%20Expression%20System%20Starter%20Kit%20with%20%20Erlenmeyer%20Flasks?unique=96c5da2)