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Chemically Defined, High-Yield Medium for Sf9 Insect Cell Culture
Gibco™ ExpiSf™ CD Medium is a GMP-manufactured, chemically defined, yeastolate-free, serum-free, and animal origin-free insect cell culture medium optimized for the high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 cells (Spodoptera frugiperda). As a key component of the ExpiSf™ Expression System, it supports recombinant protein expression at levels significantly higher than traditional insect expression platforms.
Key Features and Benefits
-
High-Yield Protein Expression
Enables 3× higher recombinant protein yield per liter than conventional serum-free insect culture media. -
Supports High Cell Densities
Allows Sf9 cells to reach densities of up to 2 × 10⁷ cells/mL in suspension culture, enhancing productivity. -
Versatile Compatibility
Suitable for culture volumes from 24-deep well plates to >2 L shake flasks without re-optimization. -
Ready-to-Use Liquid Format
Convenient liquid formulation with no supplementation required. Also available in dry AGT™ format for large-scale applications. -
Lot-to-Lot Consistency
Manufactured under ISO 13485 and cGMP (21 CFR 820), ensuring consistent performance and regulatory compliance. -
Animal Origin-Free and Defined
Free from animal-derived components, hydrolysates, or undefined proteins, ensuring reproducibility and downstream compatibility.
Recommended Applications
- High-efficiency baculovirus expression
- Protein production for structural biology and vaccine development
- Recombinant protein and virus-like particle (VLP) production
- Small- to large-scale Sf9 cell-based workflows
- Use with ExpiSf™ Enhancer and ExpiSf™ Protein Production Kit
Specifications
| Property | Details |
|---|---|
| Medium Type | Chemically defined, protein-free, serum-free |
| Cell Compatibility | Sf9 (suspension-adapted), Sf21 (with adaptation) |
| Culture Format | Suspension (not for adherent cells) |
| Concentration | Ready-to-use, liquid format |
| Working Cell Density | Up to 2 × 10⁷ cells/mL |
| Manufacturing Quality | ISO 13485; cGMP (21 CFR 820) |
| Shelf Life | 12 months from date of manufacture |
| Storage Conditions | 2°C to 8°C (protected from light) |
| Packaging | 10 L bag (sterile) |
| Shipping Condition | Ambient temperature |
Gibco™ ExpiSf™ CD Medium sets a new standard for insect cell culture performance. With chemically defined composition and robust scalability, it enables consistent, high-yield expression of recombinant proteins across a range of research and bioproduction applications. Ideal for use with the ExpiSf™ Expression System, this medium offers the reliability, flexibility, and productivity essential for modern protein expression workflows.
User Guide: ExpiSf CD Medium
Chemically Defined, High-Yield Medium for Sf9 Insect Cell Culture
Gibco™ ExpiSf™ CD Medium is a GMP-manufactured, chemically defined, yeastolate-free, serum-free, and animal origin-free insect cell culture medium optimized for the high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 cells (Spodoptera frugiperda). As a key component of the ExpiSf™ Expression System, it supports recombinant protein expression at levels significantly higher than traditional insect expression platforms.
Key Features and Benefits
-
High-Yield Protein Expression
Enables 3× higher recombinant protein yield per liter than conventional serum-free insect culture media. -
Supports High Cell Densities
Allows Sf9 cells to reach densities of up to 2 × 10⁷ cells/mL in suspension culture, enhancing productivity. -
Versatile Compatibility
Suitable for culture volumes from 24-deep well plates to >2 L shake flasks without re-optimization. -
Ready-to-Use Liquid Format
Convenient liquid formulation with no supplementation required. Also available in dry AGT™ format for large-scale applications. -
Lot-to-Lot Consistency
Manufactured under ISO 13485 and cGMP (21 CFR 820), ensuring consistent performance and regulatory compliance. -
Animal Origin-Free and Defined
Free from animal-derived components, hydrolysates, or undefined proteins, ensuring reproducibility and downstream compatibility.
Recommended Applications
- High-efficiency baculovirus expression
- Protein production for structural biology and vaccine development
- Recombinant protein and virus-like particle (VLP) production
- Small- to large-scale Sf9 cell-based workflows
- Use with ExpiSf™ Enhancer and ExpiSf™ Protein Production Kit
Specifications
| Property | Details |
|---|---|
| Medium Type | Chemically defined, protein-free, serum-free |
| Cell Compatibility | Sf9 (suspension-adapted), Sf21 (with adaptation) |
| Culture Format | Suspension (not for adherent cells) |
| Concentration | Ready-to-use, liquid format |
| Working Cell Density | Up to 2 × 10⁷ cells/mL |
| Manufacturing Quality | ISO 13485; cGMP (21 CFR 820) |
| Shelf Life | 12 months from date of manufacture |
| Storage Conditions | 2°C to 8°C (protected from light) |
| Packaging | 10 L bag (sterile) |
| Shipping Condition | Ambient temperature |
Gibco™ ExpiSf™ CD Medium sets a new standard for insect cell culture performance. With chemically defined composition and robust scalability, it enables consistent, high-yield expression of recombinant proteins across a range of research and bioproduction applications. Ideal for use with the ExpiSf™ Expression System, this medium offers the reliability, flexibility, and productivity essential for modern protein expression workflows.
User Guide: ExpiSf CD Medium
![[A35243] Gibco™ ExpiSf9™ Cells, 1.0 x 107 cells/mL](/web/image/product.product/43799/image_512/%5BA35243%5D%20Gibco%E2%84%A2%20ExpiSf9%E2%84%A2%20Cells%2C%201.0%20x%20107%20cells-mL?unique=96c5da2)
规格
| Pack Size | 500 mL, 1000 mL, 6 x 1000 mL, 10 L bag, 20 L bag |