Invitrogen™ Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, HRP, 1mg

The Invitrogen™ Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, HRP is a highly specific and affinity-purified polyclonal secondary antibody designed to detect Guinea Pig IgG (heavy and light chains) primary antibodies in various immunoassays, including Western blot (WB), immunohistochemistry (IHC), and ELISA.

Conjugated to Horseradish Peroxidase (HRP), this secondary antibody allows for chemiluminescent, colorimetric, and fluorescence-based detection when used with appropriate substrates such as ECL (Enhanced Chemiluminescence) for WB, TMB (3,3',5,5'-Tetramethylbenzidine) for ELISA, and DAB (Diaminobenzidine) for IHC.

The antibody is highly purified through antigen-affinity chromatography to ensure low background, high specificity, and minimal cross-reactivity with immunoglobulins from other species, making it an ideal choice for research applications requiring high sensitivity and specificity.

Key Features & Benefits

✔ High Sensitivity & Low Background – Affinity-purified to ensure superior specificity and minimal cross-reactivity.

✔ HRP Conjugation for Chemiluminescent/Colorimetric Detection – Compatible with ECL, TMB, and DAB substrates for high-contrast imaging.

✔ Versatile Applications – Suitable for Western blotting, ELISA, and immunohistochemistry (IHC).

✔ Stable Lyophilized Formulation – Extended shelf life and improved stability during long-term storage.

✔ Wide Dilution Range – Can be used at 1:500 - 1:20,000, depending on application.

✔ Proven Performance – Each lot is validated for sensitivity via ELISA and specificity by immunoelectrophoresis (IEP).

Applications & Recommended Dilutions

✔ Compatible with multiple detection methods:

• ECL Western Blotting Substrates for chemiluminescent detection
• TMB (3,3',5,5'-Tetramethylbenzidine) for colorimetric ELISA
• DAB (Diaminobenzidine) for immunohistochemistry staining

✔ Specificity Validation:

• No cross-reactivity observed with Canine, Donkey, Rabbit, Goat, Rat, Mouse, Sheep, or Human IgG.

Product Specifications

Target Information

The Goat anti-Guinea Pig IgG (H+L) Secondary Antibody binds specifically to the heavy and light chains of Guinea Pig IgG, making it an essential detection reagent for various immunoassays.

• Western Blot (WB): Used for detecting Guinea Pig primary antibodies in protein analysis studies.
• Immunohistochemistry (IHC): Used in tissue staining applications for detecting Guinea Pig IgG primary antibodies.
• ELISA: Facilitates antibody-based quantification of proteins and antigens.

✔ Cross-reactivity validation ensures minimal binding to non-target species.

Reconstitution & Storage Instructions

📌 Reconstitution:

• Rehydrate the lyophilized antibody with 1.1 mL of deionized water and let stand for 30 minutes at room temperature to dissolve.
• Centrifuge to remove particulates before use.
• Prepare a fresh working dilution daily.

📌 Storage Instructions:

• Lyophilized Material: Store at 2-8°C (short-term).
• Reconstituted Antibody:
  • For long-term storage, dilute with 50% glycerol and store at -20°C as a liquid.
  • Avoid repeated freeze/thaw cycles to maintain antibody integrity.

Safety & Handling

⚠ For Research Use Only (RUO) – Not for diagnostic or clinical applications.

⚠ Wear PPE – Gloves, lab coat, and eye protection recommended.

⚠ Dispose of waste properly according to laboratory safety protocols.

Related Products & Alternative Secondary Antibodies

The Invitrogen™ Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, HRP (Catalog # A18769) is a highly specific, HRP-conjugated secondary antibody designed for Western blot, ELISA, and IHC applications. Its strong HRP conjugation, high specificity, and optimized buffer formulation ensure low background noise and high signal intensity, making it a reliable choice for protein detection assays.